Calibration of FTIR Spectrophotometer

• Check that all the connections of the instrument are proper. 
• Ensure that printer is attached to the instrument. 
• Attach sample compartment. 
• Log in the software. 
• Go to Set up > validation > System suitability. 
• The instrument may ask for gathering the configuration, then click OK. 
• System suitability set up will appear. 
• Go to checks & select only Abscissa, screen report. 
• Go to archive & set archive prefix as required & click OK. 

Resolution

• Go to Instrument > scan > Instrument > Advanced.
• Keep Apodization ‘none’ & scan speed 0.1cm/s.
• The instrument may ask for collecting new background, then click OK.
• Go to Instrument > Validate > system Suitability & click OK.
• The instrument should display ‘System suitability test pass’.
• Scroll the report from the original path for display, click print icon & take print out.
• Abscissa spectrum of polystyrene film will be displayed.
• Go to graph format icon & select the range 1600 to 1580.
• Zoomed spectrum will appear, then collect the continuous curser & label at about 1583 cm-1 & 1589 cm-1, find out %T at these wavenumbers & find out the difference between two %T.
• The difference should be not less than 12%T.
• Similarly zoom between 2840 cm-1 to 2880 cm-1 & label at 2851 cm-1 & 2870 cm-1, find out %T at these wave numbers & find out difference.
• The difference should be not less than 18%T.
• Then adjust the scale to 4000 to 450 cm-1 & take printouts.
• Verification of wave number scale.
• Go to Process > Peak table & take its print.
• Peaks (transmission minima) should be observed at the wave numbers as follows, within the tolerance limit.

Wave number  cm^-1     Minimum Tolerance cm^-1     Maximum Tolerance cm^-1

3060.0                            3058.5                                     3061.5
2849.5                            2848.0                                     2851.0
1942.9                            1941.4                                     1944.4

1601.2                            1600.2                                     1602.2
1583.0                            1582.0                                     1584.0
1154.5                            1153.5                                     1155.5
1028.3                            1027.3                                     1029.3

• Remove sampling compartment. 
• Attach the diffuse reflectance sampling accessory. 
• Collect the background with KBr. 
• Go to Set up Þ validation Þ system suitability. 
• Set up will appear. 
• Go to checks & select only Abscissa, screen report. 
• Go to archive & set archive prefix as required & click OK. 
• Go to Instrument Þ validate Þ system suitability. 
• Click OK. 
• The instrument should display ‘System suitability test passes. 
• The report will be displayed, click print icon & take print out.

Maintenance/ Repairs

Whenever the instrument is out of order, then put the tag as ‘Out of Calibration’ & get it repaired.
After repair calibrate the instrument before use.

Frequency

Once in three months.

Attachments

Enclose following attachments with the calibration report.

• System suitability check report for slide holder instrument configuration. 
• Abscissa spectrum of polystyrene film with identified peaks’ table for wave number calibration. 
• Abscissa spectrum of polystyrene film (showing %T at 2851cm-1, 2870cm-1, 1583 cm-1, 1589 cm-1). 
• System suitability check report for diffuse reflectance instrument configuration. 
• Note: Words in italics stand for instrument tabs.

CALIBRATION REPORT OF FT-IR SPECTROPHOTOMETER
Reference –				Date -
Frequency - Once in three Months.				Instrument No. -
Make –				Model –
1.0	Resolution:
		Difference Between % T at 2851 cm-1 and 2870 cm-1				Difference Between % T at 1583 cm-1 and 1589 cm-1
	Limit	NLT 18%				NLT 12%
	Actual
2.0	Verification of Wave Number Scale -
	Observed Wave Number cm-1		Minimum Tolerance cm-1				Maximum Tolerance cm-1
			3058.5				3061.5
			2848.0				2851.0
			1941.4				1944.4
			1600.2				1602.2
			1582.0				1584.0
			1153.5				1155.5
			1027.3				1029.3
3.0	System suitability after attachment of diffuse reflectance sampling accessory : Complies / Does Not Comply.
4.0	Conclusion : Calibration is satisfactory / Not satisfactory.
5.0	Next Calibration Due On :
Done  By : Date :					Checked By : Date :

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