To describe the procedure for Cleaning of Microbiology Lab.
This SOP is applicable for cleaning of Microbiology Lab.
Microbiologist - Quality Control
Manager - Quality Control
5.1 Material required
5.1.1 Disinfectant / Cleaning Solution
5.1.2 Lint free cloth, sponge or wiper
5.1.3 S.S. Pot.
5.1.5 Waste bin plastic liner.
5.2 Cleaning of floor can be done by using liquid detergent. Clean the floor with plenty of water, mop the area and allow drying.
Days Name Concentration
Monday Disinfectant 1 -------%
Tuesday Disinfectant 1 -------%
Wednesday Disinfectant 2 -------%
Thursday Disinfectant 2 -------%
Friday Disinfectant 3 -------%
Saturday Disinfectant 3 -------%
Sunday Disinfectant 3 -------%
*On the basis of disinfectant qualification study use the recommended disinfectant only.
5.4 Daily and routine cleaning
5.4.1 Procedure in gray zone (microbiological section).
188.8.131.52 Ensure that the works is not in progress
184.108.40.206 Start cleaning in the following sequence with lint free duster, damped with water or IPA
220.127.116.11.1 Outer surfaces of equipment
18.104.22.168.2 Cabinet & accessories
22.214.171.124.3 Glass pans, windows &doors.
126.96.36.199.4 Empty waste bin and replace the plastic liner.
5.4.2 Wet the floor with disinfectant and allow it to be in contact with the surface for 5 – 10 minutes and mop the floor towards the direction of nearest drain Pour the left over / excess disinfectant solution in the drain.
5.4.3 Cleaning of drains
188.8.131.52 Remove the SS cover of the drain
184.108.40.206 Clean the surroundings surface below the SS cover with scrubber cloth or mop. Ensure the removal of all dirt, clean the trap.
220.127.116.11 Flush the drain with sufficient quantity of water and add about 10 ml of concentrated disinfectant solution.
18.104.22.168 Clean the SS cover of the drain and relocate at its place.
22.214.171.124 Frequency: Once a shift or as & when required.
5.5 Weekly Cleaning
5.5.1 In addition of daily cleaning activity, clean the ceiling, walls and pendants with vacuum cleaner or lint free cloth damped with IPA 70% v/v.
5.5.2 Frequency : Once a week
5.6 Cleaning procedure in white zone (Microbiological sterility room)
5.5.1 After daily work activity, clean the area as follows.
126.96.36.199 Cleaning is carried out after completion of work activity.
188.8.131.52 Ensure, aseptic activities are not in progress
184.108.40.206 Enter in the area as per SOP.
220.127.116.11 Clean the equipment outer surfaces and pendants with a lint free wet cloth with IPA 70% v/v solution.
18.104.22.168 Clean the Laminar Air Flow surfaces with a lint free wet cloth (except filters). Ensure that the filter surface ll not be in contact with cleanings equipments and cleaning agents, during the cleaning activity.
22.214.171.124 Clean the glass pans of the windows and doors with a lint free duster
126.96.36.199 Spray the disinfectant solution on the walls starting from sterility room backwards to first change room. Allow the solution to remain in contact with the surface for 5 – 10 minutes
188.8.131.52 Mop the wall of the sterile area by moving the mop with top to bottom strokes
184.108.40.206 Put disinfectant solution on floor & mop so as to remove the dust and other foreign material. (Start from working area to first change room)
220.127.116.11 Empty out the waste bin.
18.104.22.168 Garment cubicle is cleaned once a day. Open the garment cubicle, remove all the garments kept hanging and put them for washing. Clean the inner surface then outer surface with lint free cloth damped with IPA 70% v/v.
22.214.171.124 Frequency once a day or as per required
5.5.2 Fogging of sterility testing area and air locks.
126.96.36.199 Use the disinfectant as per the schedule in concentration recommends.
188.8.131.52 Pour 3 liter of disinfectant solution in reservoir of fogging instrument 2.7 liter of disinfectant solution is recommended for 1000 cubic meter of area) or as per validate method.
184.108.40.206 Plug the pin in Mains.
220.127.116.11 Operate toggle switch for ON / OFF operation
18.104.22.168 Run the instrument in all the area till the disinfectant solution exhaust.
22.214.171.124 F requency: Once a day or as & when required.
5.7 Footwear Cleaning and Disinfections.
5.7.1 Collect the footwear.
5.7.2 Immerse them in hot water. Scrub and rinse with water to remove the dirt.
5.7.3 Take out the footwear and drain the water.
5.7.4 Immerse the footwear in the disinfectant solution for about 10-15 minutes used on the day.
5.7.5 Air dry the foot wears.
5.7.6 Keep the cleaned disinfected footwear in the respective shoe racks in the provided area.
5.7.7 Frequency in case of white zone once in daily after analysis and in gray zone once in three days.
5.8 Cleaning and Disinfection of Shoe Rack
5.6.1 Wipe clean the outer surface of shoe rack with dry mop.
5.6.2 Clean the shoe rack from inside with dry mop.
5.6.3 Wipe the rack with a cloth damped with the disinfectant solution used for that day.
5.6.4 Frequency : Daily
6.1 SOP - Standard Operating Procedure
6.2 IPA - Isopropyl Alcohol
6.3 SS - Stainless Steal
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