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Test for Free Formaldehyde (Formaldehyde Content)


Learn hoe to determine the Formaldehyde Content by color development method.

Free Formaldehyde

Use Method A unless otherwise specified.

Method A

To 1.0 ml of a ten-fold dilution of the preparation under examination in a test-tube add 4.0 ml of water and 5.0 ml of acetylacetone reagent. Warm in a water-bath at 40° and allow to stand for 40 minutes. The solution is not more intensely colored than a reference solution prepared at the same time and in the same manner using 1.0 ml of a solution containing 0.002 per cent w/v of formaldehyde, CH2O, in place of the dilution of the preparation. The comparison should be made examining the tubes down their vertical axes.

Method B

To 1.0 ml of ten-fold dilution of the preparation under examination in a test-tube add 2.0 ml of water, 1.0 ml of a 1 percent w/v solution of phenylhydrazine hydrochloride, 0.5 ml of a 5 per cent w/v solution of potassium ferricyanide and 1.0 ml of hydrochloric acid and allow to stand for 15 minutes. The solution is not more intensely colored than a reference solution prepared at the same time and in the same manner using 1.0 ml of a solution containing 0.002 per cent w/v of formaldehyde, CH2O, in place of the dilution of the preparation.
The comparison should be made examining the tubes down their vertical axes.
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
Email: .moc.enilediugamrahp@ofni Need Help: Ask Question


2 Comments so far : Add yours...

Pushkar yeola said...

Hi Ankur,
Could you please suggest if I have to conduct the test on a textile substrate then how do I perform it ?
Awaiting for your reply.
Regards,
Pushkar

Ankur Choudhary said...

@Pushkar You can use above method to test formaldehyde.

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