SOP for Calibration of Plunger Operated Pipette

1.0 Objective

To calibrate the plunger operated pipette for reliable and accurate results.

2.0 Scope

This procedure is applicable to all plunger operated pipettes used in Q. C. department.

3.0 Responsibility

3.1 Doing: Technical Assistant
3.2 Checking: Executive /Manager

4.0 Accountability

Head of the Department

5.0 Procedure

Frequency: Every six month

5.1 On receipt of plunger operated pipette, perform calibration of the same.
5.2 Before testing, condition the pipette by pipetting purified water for five times and discard pipette tip.
5.3 Mount a new pipette tip and rinse with the purified water.
5.4 Weigh the beaker having a weight less than 4 gm on a microbalance.
5.5 Withdraw three different volume (maximum, minimum and middle of the range) of purified water and pipette out in to dry beaker which is previously weighed.
5.6 Calibrate the pipette by wt/wt and convert to ml by using standard wt/ml at the temperature of water at the time of calibration referring Annexure –II.
5.7 Repeat the above exercise for three times and record the results in Annexure-I.

5.8 Acceptance Criteria

For   20 µl

        50 µl                     =  + 0.7 %

        100 µl          

        200 µl

For  500 µl                    =  +  0.5%

       1000 µl

6.0 ABBREVIATIONS

6.1 SOP = Standard Operating Procedure
6.2 % = Percentage
6.3 gm = gram

ANNEXURE-I

Q. C. Department

CALIBRAITON OF PLUNGER OPERATED PIPETTE

1)  Adjustable Automated pipette :

Serial No.	: __________
Type	: __________
Trade Mark	: __________
Nominal Capacity	: __________

Subdivision :

2)  Damages                             :  None

                                                  :  Type of damage __________

3)  Testing condition                 : Temperature : __________°C

                                                     Balance Identification No         :

                                                     Thermometer Identification No.:

4)  Calculation                           :  V = W x Z

                                                     Where V  = Actual volume

                                                                W = Weight of filled water in gm

                                                                 Z = Factor in relation to temperature (Annexure II)

5)  Evaluation 

	Min				Middle				Max
	1	2	3	Mean	1	2	3	Mean	1	2	3	Mean
Measured volume
Wt. of water
Volume of water
Tolerance

• Calibration passed (within Tolerance limit)
• Calibration not passed (not within Tolerance limits)

Calibration done By :_____________                          Checked By:______________

                                     (Sign & Date)                                                    Sign & Date)

ANNEXURE-II

Q. C. Department 

CALIBRATION OF PLUNGER OPERATED PIPETTE 

FACTOR "Z" [ml/g]

Medium atmospheric pressure range 1000 to 1020 hPa
Testing Temperature [oc]	Z [ml/g]
15	1.00202
15.5	1.00209
16	1.00216
16.5	1.00224
17	1.00232
17.5	1.00240
18	1.00248
18.5	1.00257
19	1.00266
19.5	1.00275
20	1.00285
20.5	1.00295
21.0	1.00305
21.5	1.00316
22	1.00323
22.5	1.00337
23	1.00348
23.5	1.00360
24	1.00371
24.5	1.00383
25	1.00395
25.5	1.00408
26	1.00420
26.5	1.00433
27	1.00446
27.5	1.00459
28	1.00473
28.5	1.00487
29	1.00501
29.5	1.00515
30	1.00529

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