Separate laminar air flow unit in separate room for handling of microbial cultures is a new trend in pharmaceuticals now-a-days. Before some time all microbiological activities were done under same laminar airflow bench including positive cultures, sub-culturing, sample preparation and analysis. But now it is changing to have separate area for culture handling and sample analysis.
The idea behind the separate area for these activities is the possibilities of contamination of the positive cultures in the samples causing the inaccurate results. Both of the rooms should not be separated only by single door. These may open in a common corridor but the room dedicated for culture handing should have less pressure than the corridor. It is done to prevent the entrance of the contamination from culture handling room to corridor and further possibility to enter in sample analysis room.
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Positive controls should also be transferred in incubator room through separate passbox. Pathogen and other cultures should not be opened or used in other areas of the laboratory except dedicated culture handling area, washing and decontamination area.
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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