1.0 PURPOSE
To lay down the procedure for operation of Milliflex water filtration unit.2.0 SCOPE
It is applicable to Microbiology lab.3.0 RESPONSIBILITY
Microbiology personnel4.0 ACCOUNTABILITY
Head of Department5.0 PROCEDURE
5.1 Pre-operational checks5.1.1 Check the calibration status of the instrument
5.1.2 Check the status of the sanitation cycle.
5.1.3 Check the status of the monthly calibration.
5.1.4 Ensure the head, funnel and stainless steel grid are autoclaved.
5.1.5 Check the cleaning of the instrument.
5.1.6 Use sterile membrane filter only.
5.1.7 Ensure the printer is attached properly.
5.2 Operation of milliflex
5.2.1 Install the pump head. Switch on the pump by pressing the On/Off button on the keypad the display shows
MILLIFLEX PLUS
SERIAL NO. 1234
5.2.2 Set the date with arrow key by Up and Down
DATE
5.2.3 After setting the date, set time with arrow key by Up and Down
TIME
5.2.4 Manual will appear on the screen
MANUAL
5.2.5 Press OK, tare will appear.
TARE
5.2.6 Select the sampling option with the help of arrow key
5.2.7 Enabling data export.
5.2.7.1 In the main menu, use the down arrow to scroll down to export data, press OK, the display shows
START
5.2.7.2 Press OK, the display will show
OPERATOR
5.2.7.3 Press up or down keys the display will show
ABCDEFGHIJKLMNOPQRSTUVWXYZ0123456789
5.2.7.4 Enter the name by pressing the up or down arrow to highlight a character and then press OK to select it. The selected character is displayed.
5.2.7.5 Press the up and down arrow to highlight a character selection screen again
5.2.7.6 Repeat this process for each character. The characters selected so far are displayed when OK is pressed. To delete the last character entered, press C
5.2.7.7 After entering operator press OK, the display will show
CASSETTE
5.2.7.8 Press OK, the display will show
LOT NO.
5.2.7.9 Enter the media lot No. as described in point No. 5.7.1 to 5.7.6
Increment start 1
5.2.7.10 Enter the date and time, using the up and down arrows, press OK to confirm the completed entry, in each case. The display show.
Begin
Press start
5.2.10.11 Press START. The settings just entered will be printed
5.3 Set up for manual sampling
5.3.1 In the main menu, use the up or down arrows to select sampling and press OK. The display shows.
MANUAL
5.3.2 Press OK. The display shows:
VOLUME OFF
5.3.3 Select the ON option by arrow key
VOLUME ON
5.3.4 Press OK. The display shows
MILLIFLEX 100
5.3.5 Select the milliflex 250 funnel, press the down arrow, the display shows
MILLIFLEX 250
5.3.6 Press OK, the display shows:
DENSITY (g/ml)
1.00
5.3.7 Use the up or down arrows to select the density of the liquid to be tested. Press OK the display shows.
MANUAL
MIL 250/1.00
5.4 Set up for auto sampling
5.4.1 In the main menu, use the up or down arrows to select sampling and press OK. The display shows.
MANUAL
5.4.2 Press the down arrow the once. The display shows
AUTO SAMPLING
5.4.3 Press OK. The display shows the name of the last auto sampling program selected
5.4.4 Use the up or down arrows to move through the list of programs to the required program. Press OK. The display shows
SELECT
5.4.5 To proceed with the selected program, press OK. The display shows the main program screen for the selected program. For example
MF X 100
MIL 100 / 100 ML
5.5 Use sterile forceps to place the sterile membrane filter on the pump head. Firmly press the sterile funnel onto the pump head.
Note: Make sure that the base of the funnel is touch with the top of the pump head filter support.
5.6 Pour sample into the funnel, slowly to achieve the required volume
5.7 In Manual mode, if Volume ‘ON’ has been selected, the display will show the volume filled.
5.8 In Auto Sampling mode, the buzzer will sound when the required volume has been reached.
5.9 When the required volume has been reached, stop pouring.
5.10 In Manual mode, press START to start the pump.
5.11 In Auto Sampling mode, filtration starts automatically.
5.12 In Manual mode, operate the pump until the sample has been filtered, then press START to dry out the pump head. Wait for the pump to stop.
5.13 In Auto Sampling mode, when the sample has been filtered, the pump switches automatically to drying. Wait for the pump to stop.
5.14 Remove the funnel from the pump head and remove the membrane with the sterile forceps.
5.15 Place the sterile membrane filter on the pre-incubated R2A agar plates. Ensure that there is no air trap inside the filter paper
5.16 Incubates the plates as required.
5.17 Before taking next sample wipe with the head with a sporicidal agent and change the SS grid.
5.18 Calibration
5.18.1 Enter the configuration menu as described in the configuration section. Press the down arrow until the display shows validation.
5.18.2 Press OK. The display shows
WEIGHT
CALIBRATION
5.18.3 Press OK. The display shows
ENTER CODE OR
PRESS C
5.18.4 Enter the security code (press start three times) and then press OK
5.18.5 When the security code has been entered, the display shows
CALIBRATION DATE
DD MM YY
5.18.6 Press the UP or down arrows to enter the calibration date, and then press OK the display shows
REMOVE HEAD
PRESS START
5.18.7 Remove the pump head and press START, the display shows
MEASURING . . .
PLEASE WAIT . . .
5.18.8 After a few seconds the display shows
PUT HEAD
PRESS START
5.18.9 Reinstall the pump head and press START the display shows,
PUT 50 G
PRESS START 0, 0
5.18.10 Place the 50 g calibration weight on the pump head and press start
5.18.11 The pump calibrates with the 50 g weight. Next, the display prompts the user for the 100 g weight and then for the 200 g weight. Follow the prompts as they are displayed. After calibration with the three weights, the display shows,
SAVE CALIBRATION: OK
CANCEL: C
5.18.12 To proceed and confirm the calibration, press OK. The displays,
VERIFICATION
PRESS OK
5.18.13 Follow the prompts displayed to verify the new calibration. After each weight measurement, the display shows the measured value. Press START to confirm the displayed value and proceed to the next step.
5.18.14 When the verification procedure is completed, The display shows
PRINT PRESS START
CONTINUE: OK
5.18.15 Press START, the printer prints the calibration data while the display shows
PRINTING
5.18.16 When printing has finished, the display returns to
WEIGHT
CALIBRATION
Frequency of calibration: Monthly
5.19 Sanitizing
5.19.1 Sanitize the pump interior, using the automated procedure described in the Sanitization section.
Sanitizing agents: Disinfectant available for the day of use.
5.19.2 At the main program screen press OK to enter the main menu the display shows.
TARE
5.19.3 Press the down arrow until the display shows
SANITIZING
5.19.4 Press OK the display shows.
PUT EXPANDABLE
PRESS START
5.19.5 Place the sterile membrane filter and a milliflex funnel, on the pump head and press START. The display shows
FILL 100 ML OF AGENT
PRESS START
5.19.6 Fill the funnel with 100 ml of the sanitizing agent and press START. The display shows
SANITIZING
5.19.7 The pump pulls through 50 ml of the agent, pause for ten minutes, and then pulls through the remaining 50 ml A bar graph at the bottom of the display shows the progress of the operation.
5.19.8 After the pump has emptied the funnel, its runs a drying cycle and then display shows
FILL 4 X 100 ML OF WATER, PRESS START
5.19.9 Fill the funnel with 100 ml of sterile water and press START. The display shows
RINSING
5.19.10 The pump empties the funnel and runs a drying cycle then the display shows
FILL 100 ML OF WATER
PRESS START
5.19.11 Fill the funnel with 100 ml of sterile water and press START. The rinsing cycle is performed as before. Then the display prompts for the funnel to be filled again, for each rinse, for a total of four rinses when the fourth rinse has been completed, the display shows
SANITIZING FINISHED
PRESS START
5.19.12 Press START, remove the funnel and discard it, the display shows
SANITIZING
5.19.13 Press C to go back to the main program screen
Frequency of sanitization: Weekly
5.20 Cleaning
5.20.1 Clean the exterior of the pump with a lint-free mop with 70 % filtered v/v isopropyl alcohol.
Frequency: Before and after every use.
5.21 Tare procedure
Note: The tare procedure adjusts the measurement system in the pump for the weight of the installed pump head. The tare procedure shall be performed whenever the pump head is changed, and whenever the display does not show ’O’, with an empty funnel on the head.
5.21.1 When the display shows tare, press OK. The displays show
REMOVE EXPANDABLE
PRESS START
5.21.2 Remove anything on the pump head and press START. The display shows
MEASURING . . .
PLEASE WAIT . . .
5.21.3 After a few seconds, the display returns to the main program screen for the previously selected program.
5.22 Autoclave the pump head, SS grit before first use.
6.0 ABBREVIATIONS
6.1 SOP - Standard Operating Procedure
6.2 G - Gram
6.3 Ml - Milliliter
6.4 % - Percentage
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