SOP for Calibration of Infrared Spectrophotometer

1.0 OBJECTIVE

To calibrate the equipment for reliable and accurate results.

2.0 SCOPE

This procedure is applicable to the infrared spectrophotometer, installed in the quality control department.

3.0 RESPONSIBILITY

3.1 Doing: Technical Assistant
3.2 Checking: Executive /Manager

4.0 ACCOUNTABILITY

Head of the Department

5.0 PROCEDURE

Frequency of calibration: Once in a month
5.1 Operate the instrument as per SOP.
5.2 Put the reference polystyrene film (0.05 mm thick) in the sample holder and scan.
5.3 Compare the obtained spectrum with the reference spectrum for the following wave numbers as well as transmittance at shown wave number. Record the results as per annexure I.

5.3.1  Sr. No.    Wave number                                      % T

          01            1583 cm^-1 to 1589 cm^-1                       greater than 12%

          02            2851 cm^-1 to 2870 cm^-1                       greater than 18 %         

5.3.2  Sr. No.    Wave numbers of Reference Polystyrene film [Limit + 5 cm¹ ]

           1                      3027.1                                                                       

           2                      2924.0                                                                       

           3                      2850.7                                                                       

           4                      1944.0                                                                        

           5                      1871.0                                                                       

           6                      1801.6                                                                        

           7                      1601.4                                                                       

           8                      1583.1                                                                       

           9                      1181.4                                                                       

          10                     1154.3                                                                       

          11                     1069.1                                                                       

          12                     1028.0                                                                       

          13                      906.7                                                                         

          14                      698.9                                                                        

5.4 The performance of the instrument is satisfactory, if obtained I.R. Spectrum shows peaks at above wave numbers, otherwise follow the S.O.P.

6.0 ABBREVIATIONS

6.1 % = Percentage
6.2 I.R = Infra red
6.3 S.O.P. = Standard operating procedure

ANNEXURE-I

QUALITY CONTROL LABORATORY

CALIBRTAION REPORT OF INFRARED SPECTROPHOTOMETER

CALIBRATION DATE	DATE OF LAST CALIBRATION DONE	NEXT DUE FOR CALIBRATION

INSTRUMENTS DETAILS
INSTRUMENT NAME	INSTRUMENT MAKE	INSTRUMENT IDENTIFICATION

OBSERVATION

For  Transmittance  Sr.No. At wave number             % T % T  01            1583 cm-1 to 1589 cm1 greater than 12%  02  2851 cm-1 to 28701 greater than 18 %          For wave numbers    Sr. No.    Wave numbers of Reference Polystyrene film Observation Limit 01 3027.1 + 5 cm-1 02 2924.0 03 2850.7 04 1944.0 05 1871.0 06 1801.6 07 1601.4 08 1583.1 09 1181.4 10 1154.3 11 1069.1 12 1028.0 13 906.7 14 698.9