Apr 17, 2021

SOP for Handling of Spillage of Solid/Liquid Media Containing Growth

Standard operating procedure for spillage of solid and liquid media in which growth observed during microbiological testing.

1.0 Objective

To ensure the removal of the microorganisms from the surface without any health hazard.

2.0 Scope

This procedure is applicable for spillage of solid and liquid media in which growth observed during microbiological testing.

3.0 Responsibility

3.1 Doing: Technical Assistant
3.2 Checking: Executive /Manager

4.0 Accountability

Head of the Department

5.0 Procedure

5.1 Upon spillage of the solid or liquid media containing growth, wear surgical gloves and collect solid media and remove excess fluid from the surface with the help of cotton piece.
5.2 Spray 70% Isopropyl alcohol and leave it for 5 minutes.
5.3 Mop the surface twice using cotton piece with 2.5 % Dettol or 1% Hexita or 10 % Aldekol -H.
5.4 Let it dry.
5.5 Discard the media with cotton piece as per S.O.P.

6.0 ABBREVIATIONS

6.1 S.O.P.= Standard operating procedure
6.2 %= Percentage
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