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SOP for Disposal of Microbial Culture Media and Cleaning of Glassware Used for Culture Media

Standard operating procedure to dispose off the used culture media having microbial growth and cleaning of glassware used in media containing growth.


To lay down the procedure for Disposal of microbial culture media.

2.0 SCOPE:

This SOP shall be applicable to Quality Control Dept.




Head Quality Control Department.


5.1 For Culture Media Tubes/Flasks/Plates/Pipettes:

5.1.1 Autoclave the media tubes/ flasks/plates/pipettes at 121°C for 30 minutes at 15 lbs. pressure
5.1.2 Drain the contents of the tubes/ flasks/plates/pipettes into the sink under running tap water.
5.1.3 Soak the empty test tubes, pipettes, petri­plates for 1 hour in 2.0% Savlon solution.
5.1.4 Wash thoroughly with 0.1 % Teepol solution and rinse with tap water. Finally rinse with purified water and dry.

5.2 Traceability Of Disposal Load Numbers: 

5.2.1 Each disposal load is assigned a specific lot number as 7 characters as given below.
XXX indicates Serial no. of Sterilization load
YY indicates Month
ZZ indicates Year
Use of protective aprons, gloves and nose masks is essential while handling material that has been sterilized for disposal.
Run an empty Autoclave sterilization cycle at 15 lbs for 15 minutes after the sterilization of used media.
Record the disposal of microbial culture media as a “Disposal Load “ in the separate record for disposal of microbial culture media.
Assign separate load number for the disposal load.


6.1 QC : Quality Control
6.2 Dept. : Department
6.3 SOP : Standard Operating Procedure

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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