Get the latest updates from us for free
Android App

SOP for Calibration of Glassware


Standard operating procedure to calibrate the volumetric glassware used in Pharmaceutical analysis.

1.0  OBJECTIVE

       To describe the procedure for calibration of glassware.

2.0  SCOPE

       This SOP is applicable for calibration of glassware.

3.0  RESPONSIBILITY

       Officer/ Executive  - Quality Control

4.0  ACCOUNTABILITY

       Manager – Quality Control  

5.0  PROCEDURE

5.1  Volumetric glassware’s are calibrated at 23° to 27°C.
5.2  Pharmacopoeal assay and other tests involving volumetric measurements require the use of accuracy-calibrated glassware.
5.3  Clean the glassware for calibration to remove any grease, dirt etc. by keeping it immersed in chromic acid for 30 minutes.
5.4  Rinse the glassware under running tap water then rinse it twice with de-ionized water and dry it at 60-65°C for 60 minutes. Ensure that glassware is completely dry.
5.5  Remove the glassware from the oven and keep it in desiccators, ensure that the ambient temperature is 25°C ± 2°C.
5.6  Arrange a water bath consisting of plastic beaker contain water at 25°C ± 2°C.
5.7  Maintain the temperature of water bath at 25°C ± 0.5°C throughout the calibration.
5.8  Place the apparatus to be calibrated on the balance and note the weight (A), remove the apparatus and transfer the distilled water at 25°C so that the meniscus of liquid in the apparatus on the mark level. Weight the apparatus and note down the weight (B).
5.9  Calculate the volume of glass ware using following formula i.e. (B – A)/ 0.99602 
5.10  Take the volumetric flask for calibration (10 to 1000 ml)
5.11  Take pipette for calibration (1 to 50 ml)
5.12  Volumetric Flask:
         Nominal Capacity, (ml)           10         25         50         100       200       250       500         1000
         Tolerance         ±    (ml)          0.02      0.03      0.04      0.06      0.08      0.10      0.15         0.20
         (e.g., for 10 ml volumetric flask)
         Wt taken of volumetric flask = W gm
         Wt of volumetric flask + water (upto the mark level)= W1 gm
         Wt of water  = W1 – W gm
5.11  One Mark Pipette (bulb pipette)
         Nominal Capacity,  (ml)            1          2           5          10         20         25         50
         Tolerance        ±     (ml)          0.01     0.01     0.02      0.02      0.03      0.03      0.04
5.12  Graduated Pipette
         Nominal Capacity,  (ml)             1           2            5          10         25
         Sub division            (ml)           0.01      0.02      0.05       0.1        0.1                       
         Tolerance        ±     (ml)           0.01      0.01      0.02      0.02      0.03
5.13  Burette
         Nominal Capacity,  (ml)         25         50
         Sub division            (ml)        0.05      0.1                       
         Tolerance        ±     (ml)        0.03      0.05

6.0  ABBREVIATIONS

6.1  SOP - Standard Operating Procedure
6.2  Ml - Milliliter
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
Email: .moc.enilediugamrahp@ofni Need Help: Ask Question


Be the first to comment!

Post a Comment