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SOP for Calibration of Atomic Absorption Spectrophotometer (AAS)


Standard operating procedure to evaluate the performance of the Atomic Absorption Spectrophotometer (AAS).

1.0  Objective

       To check performance of the equipment for reliable and accurate results.

2.0  SCOPE

       This procedure is applicable to check performance of the atomic absorption spectrophotometer installed in quality control department.

3.0  Responsibility

3.1  Doing      : Technical Assistant
3.2  Checking : Executive /Manager

4.0  Accountability

       Head of the Department

5.0  Procedure

       Frequency : Once in a month
5.1  Operate the instrument as per respective standard operating procedure.
5.2  Use Cu standard for radiations.
5.3  Prepare 0.5 mcg/ml, 1.0 mcg/ml, 1.5 mcg/ml, 2.0 mcg/ml and 2.5 mcg/ml in water.
5.4  Aspirate the above solution and measure the absorbance.
5.5  Calculate the correlation coefficient by the instrument/computer.
5.6  Correlation coefficient should not less than 0.9900 %.
5.7  The performance of the instrument is satisfactory if the obtained correlation coefficient is within the given limit otherwise follow the S.O.P.

6.0  ABBREVIATIONS

6.1  % = Percentage
6.2  mcg= microgram
6.3  ml= millilitre
6.4  cu= copper


ANNEXURE-I
Q. C. Department
PERFORMANCE CHECK REPORT OF ATOMIC ABSORPTION
PERFORMANCE CHECK  DATE

DATE OF LAST PERFORMANCE CHECK DONE
NEXT DUE FOR
PERFORMANCE CHECKE




INSTRUMENT DETAILS
INSTRUMENT NAME

INSTRUMENT MAKE

INSTRUMENT
IDENTIFICATION NO.





OBSERVATION


Test % w/v
Absorption
Mean

1
2
3

0.5 mcg/ml




1.0 mcg/ml




1.5 mcg/ml




2.0 mcg/ml




2.5 mcg/ml






 Correlation  coefficient : _________ (Limit : Not less than 0.9900 %)
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
Email: .moc.enilediugamrahp@ofni Need Help: Ask Question


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