1.0 Objective
To sterilize the accessories by dry heat sterilization.2.0 Scope
This procedure is applicable to all accessories which are to be sterilized by DHS oven.3.0 Responsibility
3.1 Doing : Technical Assistant/(microbiologist)3.2 Checking : Executive /Manager
4.0 Accountability
Head of the Department5.0 Procedure
5.1 List of accessoriesGlass wares
Lid & slit of air sampler
Forceps
S.S cap/Tray
Cutter
5.2 Wash the glass wares as per the S.O.P.
5.3 Put the accessories in DHS oven or in the allocated S.S. containers in DHS oven.
5.4 Put the whole container in open condition in the oven for dry heat sterilisation.
5.5 Affix the chemical indicator on container / Accessories.
5.6 Operate the DHS oven as per S.O.P.
5.7 Sterilise the accessories at 180°C for 2 hours.
5.8 After completion of cycle close the container and take the printout.
5.9 Check the response of chemical indicator, the green colour of indicator should be change to black.
5.10 Transfer the sterile accessories with help of trolley in sterility room or inoculation room where ever required.
5.11 Use these accessories within 24 hours.
5.12 Resterilized the unused accessories after 24 hours.
5.13 Maintain the record in Annexure-I.
5.14 Attach the chart and chemical indicator along with record of Annexure-I.
6.0 ABBREVIATIONS
6.1 S.O.P.No.- Standard operating procedure number
Note. : - If the printer is out of order record the results manually.
ANNEXURE-I
MICROBIOLOGICAL DEPARTMENT
DRY HEAT STERILIZATION RECORDS
S.O.P.NO._______________Instrument Identification No.:____________________
Date
|
Cycle No.
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Accessories to be sterilized
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Sterilization Instruction
|
DHS Cycle started at
|
DHS Cycle completed at
|
Chemical Indicator response
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Sign.
|
Remarks
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180°C for
2 hours
| ||||||||
180°C for
2 hours
| ||||||||
180°C for
2 hours
| ||||||||
180°C for
2 hours
|
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