Learn how High Performance Liquid Chromatography works and how chromatographic column separates the compounds from a mixture.
Related: HPLC Column Performance Evaluation and Column Care
Related: Guideline for Preparation of Mobile Phase
Related: Resolution Factor, Tailing Factor, Theoretical Plates and Capacity Factor in HPLC
Detectors are used to determine the separated compounds by ultra violet absorption. The light absorption depends upon the concentration of compound in mobile phase. These signals are recorded by the computer software in the form of peaks and purity of compound is calculated by peak area of different compounds.
Also see: Calibration of HPLC
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