SOP for Plate Exposure

1.0 OBJECTIVE

To lay down the procedure for plate exposure in areas which have been fumigated, in order to determine the efficacy of fumigation.

2.0 SCOPE

This SOP shall be applicable to Quality Control Dept.

3.0 RESPONSIBILITY

Microbiologist

4.0 ACCOUNTABILITY

Sr. Manager Quality Assurance

5.0 PROCEDURE

5.1 Prepare sterile plates of Nutrient Agar as per the procedure described in SOP for media preparation on the day of fumigation or prior to fumigation.
5.2 Preincubate the plates at 30 - 35° C for 24 hours, before use.
5.3 Stack the preincubated Petri dishes in a stainless steel canister and take the canister to the fumigated area.
5.4 Before exposing the plates, ensure that the airflow of the fumigated area has been switched on and the area is free of the fumes. Expose two plates at two ends of the fumigated room
5.5 Remove the plate from the canister and take it to the point of exposure.
5.6 Remove the lid of the plate and place it beside the plate, in an inverted position.
5.7 Expose the plate for a period of 30 minutes.
5.8 At the end of 30 minutes, place the lid back on the plate and mark the plate, mentioning the date and point of exposure.
5.9 Put the plate back into the canister.
5.10 Incubate the plate at 30 - 35° C for 5 days.
5.11 Count the number of colonies at the end of 5 days.
5.12 The count should not exceed 100 cfu/plate.
5.13 If the count exceeds 100, it indicates that fumigation was ineffective.
5.14 In this case, re-fumigate the area and repeat the entire procedure described above.

6.0 ABBREVIATIONS

6.1 SOP: Standard Operating Procedure
6.2 CFU: Colony Forming Unit
6.3 QC: Quality Control
6.4 Dept.: Department

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