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SOP for Fumigation of Microbiology Laboratory


Standard operating procedure of fumigation process in microbiology laboratory and precaution taken during the fumigation.

1.0 OBJECTIVE:

      To lay down the procedure for fumigation of Microbiology Laboratory.

2.0 SCOPE:

      This SOP shall be applicable to Quality Control Dept.

3.0 RESPONSIBILITY: 

      Microbiologist

4.0 ACCOUNTABILITY:

      Head Quality Control Department.

5.0 PROCEDURE:

5.1 Apparatus:

Fumigation machine

5.2 Reagent:

5 % Gramicid Solution

5.3 Fumigation Procedure:

Frequency: Once in a week or if the microbial counts exceed the limits in Microbiology Testing area .
5.3.1 Connect the machine electric plug to main supply.
5.3.2 Keep the machine 2 feet above the ground level.
5.3.3 Open the lid of tank.
5.3.4 Add 2 ltrs of 5 % Gramicid solution and close the lid.
5.3.5 Adjust the blower at approximately 45° angle.
5.3.6 Put on the MAIN switch.
5.3.7 Switch on the blower.
5.3.8 Fogger starts emitting fine fog.
5.3.9 Keep the fog generator control minimum, to get fine particles of gramicid solution.
5.3.10 Keep the fogger on till it forms sufficient fog in the area, and then shift to another area.
5.3.11 AHU should be put OFF before starting fumigation.
5.3.12 After fumigation keep all the doors closed.
5.3.13 After completion of fumigation keep the area closed for not less than 6 hrs.
5.3.14 Switch on the AHU 1 hour before starting the work for defumigation
5.3.15 Maintain fumigation Record.

5.4 Precautions:

5.4.1 Make sure the area to be fumigated cleaned thoroughly.
5.4.2 Cover the electronic equipments with plastic sheet.
5.4.3 AHU should be put OFF 10 minutes before the fumigation starts.
5.4.4 AHU can be put ON 1 hour before starting work.
5.4.5 Use proper safety apparel such as gowns, rubber hand gloves and safety goggles during fumigation.

6.0 ABBREVIATIONS:

6.1 SOP : Standard Operating Procedure
6.2 Dept. : Department
6.3 AHU : Air Handling Unit



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