SOP for Disposal of Microbial Culture Media and Cleaning of Glassware Used for Culture Media

1.0 OBJECTIVE

To lay down the procedure for Disposal of microbial culture media.

2.0 SCOPE

This SOP shall be applicable to Quality Control Dept.

3.0 RESPONSIBILITY

Microbiologist.

4.0 ACCOUNTABILITY

Head Quality Control Department.

5.0 PROCEDURE

5.1 For Culture Media Tubes/Flasks/Plates/Pipettes

5.1.1 Autoclave the media tubes/ flasks/plates/pipettes at 121°C for 30 minutes at 15 lbs. pressure
5.1.2 Drain the contents of the tubes/ flasks/plates/pipettes into the sink under running tap water.
5.1.3 Soak the empty test tubes, pipettes, petri­plates for 1 hour in 2.0% Savlon solution.
5.1.4 Wash thoroughly with 0.1 % Teepol solution and rinse with tap water. Finally rinse with purified water and dry.

5.2 Traceability Of Disposal Load Numbers 

5.2.1 Each disposal load is assigned a specific lot number as 7 characters as given below.
XXX/YYZZ
XXX indicates Serial no. of Sterilization load
YY indicates Month
ZZ indicates Year

NOTE:

• Use of protective aprons, gloves and nose masks is essential while handling material that has been sterilized for disposal.
• Run an empty Autoclave sterilization cycle at 15 lbs for 15 minutes after the sterilization of used media.
• Record the disposal of microbial culture media as a “Disposal Load “ in the separate record for disposal of microbial culture media.
• Assign separate load number for the disposal load.

6.0 ABBREVIATIONS

6.1 QC: Quality Control
6.2 Dept.: Department
6.3 SOP: Standard Operating Procedure

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