Calibration of Automatic Potentiometric Titrator

Turn the instrument & printer on.

Repeatability of Factor Determination

Go to titer mode.
Use Disodium tartrate as standard.
Take all precautions to avoid variations due to weighing & transfer of standard.
Enter sample weight.
Find out the factor.
Take five readings.
Calculate %RSD & average of five.
%RSD of five readings should be less than 0.5.

Calibration of Burette

Clean the ICB (Interchangeable burette).
Fill water in the reservoir.
Fill the burette.
Go to AUX functions & set Eject volume to 0.10ml.
Quit & then press Eject.
Collect the volume in clean, dry, & previously weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 0.0990ml to 0.1010 ml.
Fill the burette.
Go to AUX functions & set Eject volume to 1.0ml.
Quit & then press Eject.
Collect the volume in clean, dry, & previously weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 0.99ml to 1.01 ml.
Fill the burette.
Go to AUX functions & set Eject volume to 10.0ml.
Quit & then press Eject.
Collect the volume in clean, dry, & previously weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 9.98ml to 10.02 ml.
Fill the burette.
Go to AUX functions & set Eject volume to 20.0ml.
Quit & then press Eject.
Collect the volume in a clean, dry, & pre-weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 19.97ml to 20.03 ml.

Precautions

Clean the ICB with acetone after calibration of burette is over.

Frequency

Once in three months.

REFERENCE

User’s guide for Automatic Potentiometric Titrator
Indian Pharmacopoeia

A]  Repeatability of factor determination
Sr. No.		Burette Reading (in ml.)			Factor				Average		% RSD
1
2
3
4
5
ACCEPTANCE CRITERIA % RSD Not more than 0.5%.
B]  Calibration of Burette.
S. No.	Volume (ml.)		I	II			III	IV		Limit (ml.)
			Empty Beaker Wt. (g.)	Wt. of Beaker + Water (g.)			II –I	III / 0.99602
1	0.1									0.0990 to 0.1010
2	1.0									0.99 to 1.01
3	10.0									9.98 to 10.02
4	20.0									19.97 to 20.03
CONCLUSION – Calibration Complies / Does not comply as per Standard Calibration Procedure.
Next Calibration Due On -
Done By : Date :						Checked By : Date :