SOP for Cleaning of High Performance Thin Layer Chromatograph

1.0 OBJECTIVE

To clearly lay down the procedure for cleaning.

2.0 SCOPE

This procedure is applicable to High-performance thin layer chromatography, installed in API quality control laboratory.

3.0 RESPONSIBILITY

3.1 Doing: Technical Assistant /Executive
3.2 Checking: Executive/Manager

4.0 ACCOUNTABILITY

Head of the Department

5.0 PROCEDURE

5.1 Clean completion of the analysis do the following.
5.2 Clean the syringe with the respective solvent to remove any residue.
5.3 Clean the syringe properly with distilled water, if required sonicate for 10 minutes in distilled water.
5.4 Clean the chamber of high-performance thin layer chromatography with raw water and then with purified water.
5.5 Finally clean the chamber with methanol.
5.6 Clean with tissue paper.
5.7 Dry the tank with a dryer.
5.8 Kept the tank with lid at an allocated place.
5.9 Clean the outer side of linnomate, scanner and U.V cabinet with a dry clean cotton cloth.
5.10 Remove any dirt or spot by mopping of isopropanol.
5.11 Dry it with tissue paper.
5.12 Remove any used TLC plate from the U.V.cabinet.
5.13 Record in the instrument log card.

6.0 ABBREVIATIONS

6.1 TLC = Thin Layer Chromatography
6.2 U.V = Ultraviolet
6.3 API = Active Pharmaceuticals Ingredient

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