1.0 OBJECTIVE
To calibrate the equipment for reliable and accurate results.2.0 SCOPE
This procedure is applicable to the UV Cabinet, installed in the quality control department.3.0 RESPONSIBILITY
3.1 Doing: Technical Assistant3.2 Checking: Executive/ Manager
4.0 ACCOUNTABILITY
Head of the Department5.0 PROCEDURE
5.1 Frequency: Once in 3 months.5.2 For 254 nm
5.3 Operate the instrument as per S.O.P.
5.4 Prepare a 0.04% w/v solution of sodium salicylate in ethanol (95%)
5.5 Apply 5µl of this solution on Silica gel G with a diameter of about 5mm and examine visually.
5.5 The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
5.7 The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.
5.8 For 366 nm
5.9 Frequency: Once in 3 months
5.10 Operate the instrument as per S.O.P.
5.11 Prepare a 0.2% w/v solution of sodium salicylate in ethanol (95%)
5.12 Apply 5µl of this solution on Silica gel with a diameter of about 5mm and examine visually.
5.13 The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
5.14 The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.
Related: SOP for UV Cabinet
6.2 Q.C. = Quality Control
6.3 TLC plate = Thin layer chromatographic plate.
6.4 % w/v = Percentage weight by volume.
6.5 mm = millimetre
Wt. of Sodium salicylate taken :-________________ mg (About 0.04 gm) dilute to100 ml with ethanol (95%).
6.0 ABBREVIATION
6.1 UV Cabinet = Ultra violet Cabinet6.2 Q.C. = Quality Control
6.3 TLC plate = Thin layer chromatographic plate.
6.4 % w/v = Percentage weight by volume.
6.5 mm = millimetre
Wt. of Sodium salicylate taken :-________________ mg (About 0.04 gm) dilute to100 ml with ethanol (95%).
Spotting: Diameter of about 5mm and volume of 5μl and examine visually at 254nm
Results: The lamp should be capable of revealing, without doubt, a standard spot of sodium salicylate.
Results: The lamp should be capable of revealing, without doubt, a standard spot of sodium salicylate.
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