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SOP for Calibration Procedure of U.V.Cabinet


Standard operating procedure to evaluate the performance of UV Cabinet for reliable and accurate results using sodium salicylate solution.

1.0  OBJECTIVE

       To calibrate the equipment for reliable and accurate results.

2.0  SCOPE

       This procedure is applicable to the UV Cabinet, installed in quality control department.

3.0  RESPONSIBILITY

3.1  Doing      :  Technical Assistant
3.2  Checking :  Executive/ Manager

4.0  ACCOUNTABILITY

       Head of the Department

5.0  PROCEDURE

5.1  Frequency : Once in 3 months.

5.2  For 254nm

5.3  Operate the instrument as per S.O.P.
5.4  Prepare a 0.04% w/v solution of sodium salicylate in ethanol (95%)
5.5  Apply 5µl of this solution on Silica gel G with a diameter of about 5mm and examine visually.
5.5  The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
5.7  The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.

5.8  For 366nm

5.9  Frequency: Once in 3 months
5.10  Operate the instrument as per S.O.P.
5.11  Prepare a 0.2% w/v solution of sodium salicylate in ethanol (95%)
5.12  Apply 5µl of this solution on Silica gel with a diameter of about 5mm and examine visually.
5.13  The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
5.14  The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.

6.0  ABBREVIATION

6.1  UV Cabinet = Ultra violet Cabinet
6.2  Q.C. = Quality Control
6.3  TLC plate = Thin layer chromatographic plate.
6.4  % w/v = Percentage weight by volume.
6.5  mm = millimetre
      1. Check For short wave length (254nm).
      Wt. of Sodium salicylate taken :-________________ mg. 100ml with ethanol (95%).
                                                        (About 0.04 gm)
Spotting : Diameter of about 5mm and volume of 5μl and examine visually at 254nm 
Results : 
The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.



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