SOP for Operating Procedure of Colony Counter

1.0 Objective

To ensure the rapid and accurate counting of microbial colonies (cfu).

2.0 SCOPE 

This Procedure is applicable for counting of microbial colonies (cfu) from petri plates during water and environmental monitoring.

3.0 Responsibility

Doing : Tech.Assistant ( Microbiologist)
Checking : Executive/Manager

4.0 Accountability

Head of the Department

5.0 Procedure 

5.1 Clean the instrument with dry clean cotton cloth.
5.2 Switch ‘ON’ the main power supply.
5.3 Switch ‘ON’ the instrument.
5.4 Connect plug of the Auto Marker Pen to the probe socket provided on instrument.
5.5 Keep the petri dish on the Wolffhuegel glass of instrument.
5.6 Adjust the magnifier lens with the help of adjustable knob as convenient.
5.7 Switch ‘ON’ the illumination.
5.8 Press the ‘RESET’ button. The display will show 0000
5.9 Press the ‘AUDIO’ button.
5.10 Select the square of Wolffhuegel grid one by one and mark the colonies with the help of Auto Marker Pen.
5.11 The marking ensures counting of colonies and audio beep indicates that the count has been made.
5.12 Marking of all colonies can be observed through magnifier lens and counting should be done simultaneously which is shown on display.
5.13 Record the total number of cfu count from display in respective annexure.
5.14 Switch ‘OFF’ the illumination

5.15 Switch ‘OFF ‘ the instrument.
5.16 Switch ‘OFF’ the main power supply.
5.17 Clean the instrument with clean, cotton cloth.

6.0 ABBREVIATIONS

6.1 SOP - Standard Operating Procedure
6.2 Cfu - Colony forming Unit

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