High Performance liquid Chromatography also known as HPLC is a widely used instrument for analysis in pharmaceutical, chemical and food industries. It has powerful ability to identify and quantify the complex mixtures and this makes it an essential instrument for analytical laboratories. This article will provide detailed knowledge on major components, working and principle of HPLC.
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Relative Response Factor (RRF) and its Calculation in HPLC Analysis
During the manufacturing process of active pharmaceutical ingredients, some unwanted substances are produced those are known as impurities. Some impurities are raised due to the degradation of the actual drug.
Steps for HPLC Method Development
Analytical method development is considered as a critical process in pharmaceuticals. Availability of the different types of columns, operating parameters, mobile phase composition, diluent and pH values make it critical to develop an analytical method. A good analytical method should be simple, used column, mobile phase and buffer should be common. It can be done easily step by step.
HPLC System Software Validation Plan
Introduction
Softwares are used to operate and interpret the analysis results in HPLC systems. There are many softwares those are used in different HPLC systems. All softwares are required to validate to ensure the accuracy of the produced data.
Software Validation is a requirement of GxP system. This software validation is intended to provide details of activities carried out and results therei…
Software Validation is a requirement of GxP system. This software validation is intended to provide details of activities carried out and results therei…
Steps for HPLC Method Validation
In the pharmaceutical industry, validation is an essential part of quality control and quality assurance. Various regulatory authorities give particular emphasis on the validation of all the processes used in the industry. Validation is a formal and systematic way to demonstrate the suitability of the assay method to provide useful data to ensure that the process or the technique gives satisfacto…
Difference between C8 and C18 Columns Used in HPLC System
High Performance Liquid Chromatography system (HPLC) refers to a technique in analytical chemistry that is used to identify, separate and quantify each component in a mixture. C8 and C18 both refer to the alkyl chain of a bonded face of a column. They are both used in the high performance liquid chromatography system. The two columns have some similarities, but they are also different in some way…
10 Tips for HPLC Analysis In Pharmaceuticals
HPLC, also known as High-Performance Liquid Chromatography system, is one of the most crucial analytical methods in pharmaceuticals. The purpose of HPLC is the separation of each compound that make up a mixture.
It passes pressurized liquid solvent with the use of pumps which holds the sample mixture via a column full of solid adsorbent substance. There is a difference in the flow rates for each …
It passes pressurized liquid solvent with the use of pumps which holds the sample mixture via a column full of solid adsorbent substance. There is a difference in the flow rates for each …
Insight on Data Integrity in Chromatography
The core objective of any analytical work is to produce reliable and scientifically accurate data. When the process is compromised the analyst is bound to obtain inaccurate results. This, in turn, affects the integrity of the data. Data integrity in the chromatography analysis is a key area of concern in the pharmaceutical industry. Technicians have linked it to the ease of data manipulation when…
System Suitability in HPLC Analysis
HPLC, short for High-performance liquid chromatography is a technique used for separating the components in a mixture. In the HPLC technique, a liquid sample is passed over an absorbent material to test its efficacy.
HPLC chromatography technique is used in pharmaceutical industries for research and testing purposes. Most of the pharmaceutical companies follow a three-step approach to check the f…
HPLC chromatography technique is used in pharmaceutical industries for research and testing purposes. Most of the pharmaceutical companies follow a three-step approach to check the f…
Different Types of HPLC Detectors
HPLC detectors are used in the detection of the solute present in the eluent coming from the HPLC column. They are capable of determining the identity and concentration of eluting compounds in the mobile phase. There are specific detectors which respond to a specific compound and their response is not dependent on mobile phase composition.
Extraneous Peaks in Chromatographic Analysis
Chromatography is a process that allows you to separate compounds based on their affinity to different properties. This separation begins with a mixture of compounds and ends with individual peaks or bands of each compound on its own graph. Compounds can interfere with chromatography in many ways, causing peaks that shouldn’t be there. These extraneous peaks ruin the clarity of your results and m…
HPLC Calibration Procedure
Check HPLC chromatography (Pump) for the following:
I. Checkpoint: Leakage test (By Pressure Drop)
II. Flow rate calibration
CHECKPOINT: LEAKAGE TEST (BY PRESSURE DROP) 1. Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2. Place inlet tubing of the Pump into the Water HPLC grade through a suction filter. 3. Allow the mobile phase to flow for about 5 min.
…
I. Checkpoint: Leakage test (By Pressure Drop)
II. Flow rate calibration
CHECKPOINT: LEAKAGE TEST (BY PRESSURE DROP) 1. Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2. Place inlet tubing of the Pump into the Water HPLC grade through a suction filter. 3. Allow the mobile phase to flow for about 5 min.
…
