Working with hazardous chemicals can have a serious risk to health if are used incorrectly and without the necessary training or safety precautions. In this post, I will give you guidance on how to work with these hazardous materials safely so that you can be confident working with these materials in your laboratory.
Showing posts with label Reagents. Show all posts
Preparation of Buffer Solutions | Phosphate, Acetate and other Buffers
Buffer solutions play an important role in ensuring product stability and providing environment for chemical and biochemical reactions. Buffers are used in analytical methods, formulation development and stability studies of products. It is essential to understand how to prepare buffer solutions correctly to achieve reliable results in laboratory and manufacturing of pharmaceutical products. This…
SOP for Preparation and Standardization of Volumetric Solution
1.0 OBJECTIVE
To describe the procedure for preparation and standardization of volumetric solutions.
2.0 SCOPE This SOP is applicable for preparation and standardization of volumetric solutions.
3.0 RESPONSIBILITY Officer/ Executive - Quality Control.
4.0 ACCOUNTABILITY Manager – Quality Control.
5.0 PROCEDURE 5.1 Volumetric solutions shall be standardized by titration against a primary standard…
2.0 SCOPE This SOP is applicable for preparation and standardization of volumetric solutions.
3.0 RESPONSIBILITY Officer/ Executive - Quality Control.
4.0 ACCOUNTABILITY Manager – Quality Control.
5.0 PROCEDURE 5.1 Volumetric solutions shall be standardized by titration against a primary standard…
SOP for Preparation of Reagent Solution
1.0 OBJECTIVE
To lay down the procedure for preparation of Reagent solutions.
2.0 SCOPE This SOP shall be applicable to Quality Control Dept.
3.0 RESPONSIBILITY Quality Control Supervisor
4.0 ACCOUNTABILITY Sr. Manager Quality Assurance
5.0 PROCEDURE 5.1 The solution shall be prepared by the chemist who actually does the analysis. The solution shall be prepared when it is required.
5.2 The quant…
2.0 SCOPE This SOP shall be applicable to Quality Control Dept.
3.0 RESPONSIBILITY Quality Control Supervisor
4.0 ACCOUNTABILITY Sr. Manager Quality Assurance
5.0 PROCEDURE 5.1 The solution shall be prepared by the chemist who actually does the analysis. The solution shall be prepared when it is required.
5.2 The quant…
Preparation of Molar and Normal Solutions
Molar and
normal solutions are used in the chemical analysis of raw materials. A specific
amount of chemical (solute) is dissolved to obtain the solution of required concentration
(molar or normal solutions).
Karl Fischer Reagent and Its Reactions
You are given a sample with a trace amount of water and you need to determine the amount of water in the sample, what do you do? The method to use is the Karl Fischer (KF) moisture analysis. Karl Fischer method uses the Karl Fischer Reagents to determine the moisture content of a sample and it is a widely popular method because it allows you to determine the water content of drug in a very fast, …
Determination of Shelf Life of Solutions in Laboratory
Incorrect strength or concentration of volumetric solutions, reagents and buffer solutions can alter the results of analyzed products. Solutions prepared for chemical analysis are not stable for a longer period.
Preparation and Standardization of 0.1 M Silver Nitrate
Silver Nitrate Solution PreparationTake about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.Add about 17 gm of Silver Nitrate with continues stirring. Add more about 700 ml of distilled water, mix.Make up the volume 1000 ml with water. Mix solution thoroughly. Keep the solution for at least one hour and then carry out the standardization.
Related: Determination of Shelf Life of…
Preparation and Standardization of 0.1 M Sodium Thiosulphate
Sodium Thiosulphate Solution PreparationTake about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.Add about 25 gm of Sodium Thiosulphate with continues stirring.Add about 0.2 gm of Sodium Carbonate with continues stirring.Add more about 700 ml of water, mix.Make up the volume 1000 ml with water. Mix solution thoroughly.Keep the solution for at least one hour and then carry out t…
Preparation and Standardization of 0.1 M Hydrochloric acid (HCl)
Hydrochloric acid Solution PreparationTake about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.Add about 8.5 ml of Conc. Hydrochloric acid with continuous stirring. Add more about 700 ml of water, mix and allow to cool to room temperature. Make up the volume 1000 ml with water. Mix solution thoroughly. Keep the solution for at least one hour and then carry out the standardizati…
Preparation and Standardization of 0.1 N Perchloric acid
Perchloric acid Solution PreparationTake about 500 ml of anhydrous glacial acetic acid & about 25 ml Acetic anhydride in a cleaned and dried 1000 ml volumetric flask.Add about 8.5 ml of Perchloric acid (About 70%) with continues stirring.Cool the solution.Make up the volume 1000-ml with anhydrous glacial acid. Mix solution thoroughly.Keep the solution for at least (24 hours) for the excess a…
Preparation and Standardization of 0.1 M Disodium Edetate (EDTA)
Disodium Edetate Solution PreparationTake about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.Add about 37.2 gm of Disodium Edetate with continues stirring. Add more about 700 ml of water mix. Make up the volume 1000 ml with water. Mix the solution thoroughly. Keep the solution for at least one hour and then carry out the standardization.
Related: Determination of Shelf Life of…
Preparation of Indicator Solutions
Indicators In the test and assays of the pharmaceutical ingredients, indicators are required to indicate the completion of a chemical reaction in volumetric analysis or to indicate the pH of solutions. Indicators may be substituted for one another provided the colors change over approximately the same range of pH but in the event of doubt or dispute as to the equivalence of indicators for a partic…
Preparation of Standard Solutions
Acetaldehyde Standard Solution (100 ppm C2H4O): Dissolve 1.0 g of acetaldehyde in sufficient 2-propanol to produce 100 ml and dilute 5.0ml of the solution to 500.0 ml with 2-propanol. Aluminium Standard Solution (2 ppm Al): Dilute 2 volumes of a 0.176 percent w/v solution of aluminium potassium sulfate in 0.1 M sulphuric acid to 100 volumes with water. Aluminium Standard Solution (10 ppm Al): Dilut…
