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SOP for Guidelines for Working in Microbiology Laboratory


Standard operating procedure to provide the guidelines of system and working in microbiology laboratiory

1.0  OBJECTIVE:

       To lay down a procedure for the guidelines for Microbiology laboratory.

2.0  SCOPE:

       This SOP shall be applicable for Microbiology laboratory.

3.0  RESPONSIBILITY:

       Microbiologist

4.0  ACCOUNTABILITY:

       Head QA & QC

5.0  PROCEDURE:

5.1  Personnel must not wear cosmetics, nail polish or jewellery.
5.2  Before entering in laboratory all people should wear cap, apron and slippers provided.
5.3  Mask and gloves are necessary for working under L.A.F. bench.
5.4  Entry in microbiology section should be restricted and only authorized personnel shall allow minimizing the microbial load.
5.5  Movement in the micro laboratory should be rhythmic and head snatching, rubbing hands, face or parts of the body must be consciously avoided.
5.6  The person with physically illness, like stomach or respiratory disorders should not enter the laboratory. If analyst is suffering from any illness it must be reported to In-charge.
5.7  All the doors of respective areas of laboratory should keep in close condition and Approved SOP shall be follow for handling of micro equipment. Analyst should not open doors of incubator rooms frequently to avoid contamination.
5.8  Cleaning of the laboratory should be done before work starts and at the end of the day. Laboratory area or equipment should not clean while work is going on in that area.
5.9  All aseptic testing should be done under Grade A (i.e. Under L.A.F.) and during aseptic work; hands should be sanitized by filtered 70% isopropyl alcohol at when ever necessary.
All waste materials must be timely and properly removed at the end of each work day from LAF.
5.10  All sterilised materials should be stored in cool, dry place under laminar units or under the area having terminal HEPA filters.
5.11  Before starting and after completion of microbiological work, wash and sanitize the hands upto forearm with medicated soap and disinfectant solution.
5.12  The used disposable caps mask and gloves should be autoclaved in autoclavable bag and then dispose.
6.0  ABBREVIATIONS:
SOP : Standard Operating Procedure
QC : Quality Control
LAF : Laminar Air Flow



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