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Calibration of Automatic Potentiometric Titrator


Learn how to calibrate the Automatic Potentiometric Titrator using Disodium tartarate as standard.
Turn the instrument & printer on.

Repeatability of factor determination.

Go to titer mode.
Use Disodium tartarate as standard.
Take all precautions to avoid variations due to weighing & transfer of standard.
Enter sample weight.
Find out the factor.
Take five readings.
Calculate %RSD & average of five.
%RSD of five readings should be less than 0.5.

Calibration of Burette.

Clean the ICB (Inter changeable burette).
Fill water in reservoir.
Fill the burette.
Go to AUX functions & set Eject volume to 0.10ml.
Quit & then press Eject.
Collect the volume in clean, dry, & previously weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 0.0990ml to 0.1010 ml.
Fill the burette.
Go to AUX functions & set Eject volume to 1.0ml.
Quit & then press Eject.
Collect the volume in clean, dry, & previously weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 0.99ml to 1.01 ml.
Fill the burette.
Go to AUX functions & set Eject volume to 10.0ml.
Quit & then press Eject.
Collect the volume in clean, dry, & previously weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 9.98ml to 10.02 ml.
Fill the burette.
Go to AUX functions & set Eject volume to 20.0ml.
Quit & then press Eject.
Collect the volume in clean, dry, & pre weighed beaker.
Weigh the beaker & find out the weight of ejected volume, further find out the volume.
Volume = Weight in g / 0.99602
Volume should be between 19.97ml to 20.03 ml.

Precautions

Clean the ICB with acetone after calibration of burette is over.
Frequency
Once in three months.
REFERENCE:
User’s guide for Automatic Potentiometric Titrator
Indian Pharmacopoeia

A]  Repeatability of factor determination

Sr. No.

             Burette Reading (in ml.)

Factor
Average
% RSD
1
2
3
4
5
ACCEPTANCE CRITERIA
% RSD Not more than 0.5%.

B]  Calibration of Burette.

    S. No.

Volume (ml.)

I

II

III

IV
Limit (ml.)

Empty Beaker Wt. (g.)

Wt. of Beaker + Water (g.)

II –I

III / 0.99602

1

0.1

 

0.0990 to 0.1010

2

1.0

 

0.99 to 1.01

3

10.0

9.98 to 10.02

4

20.0

19.97 to 20.03
CONCLUSION –
Calibration Complies / Does not comply as per Standard Calibration Procedure.
Next Calibration Due On -
Done By :
Date :
Checked By :
Date :

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
Email: .moc.enilediugamrahp@ofni Need Help: Ask Question


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