1.0 OBJECTIVETo describe the procedure for operation and calibration of Refractometer.
2.0 SCOPEThis SOP is applicable for operation and calibration of Refractometer.
3.0 RESPONSIBILITYOfficer/ Executive - Quality Control
4.0 ACCOUNTABILITYManager - Quality Control
5.1 Operations5.1.1 Ensure that the instrument is free from dust.
5.1.2 Ensure that all the parts including eye lens, prisms and mirror are clean before operation.
5.1.3 Place the instrument on a table where adequate light can be reflected through the mirror.
5.1.4 Open the prism lock and place few drops of liquid testing sample at the temperature specified.
5.1.5 Allow the liquid to smear on the lower prism surface quickly close the prism lock.
5.1.6 Adjust the eye piece cross wire with a knob by rotating it in a clockwise or anti-clockwise direction so that the sharp demarcation line passes through its center.
5.1.7 Sharp demarcation is produced in the form of a circle having half light & half dark portion.
5.1.8 A knob provided just above the prism can do fine adjustment.
5.1.9 Note down the reading from the scale with the help of the magnified lens of the instrument.
5.1.10 Use thermometer and water jacket with tubes provided in the instrument whenever it is required.
5.1.11 Clean the instrument and keep under box when not in use.
5.2 Calibration5.2.1 To achieve accuracy, the instrument should be calibrated against distilled water, which has a refractive index of 1.3325 at 25 °C against the reference liquids given below:
Carbon Tetrachloride : 1.4603
Toluene : 1.4969
Water : 1.3325
5.2.2 Frequency of calibration: Monthly.
5.3.1 Proper handling of the instrument.
5.3.2 Temperature should be carefully adjusted and maintained since refractive index varies significantly with temperature.
6.0 ABBREVIATION6.1 SOP - Standard Operating Procedure
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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