1.0 OBJECTIVETo describe the procedure for the procedure for opening of lyophilized vials of microorganisms.
2.0 SCOPEThis SOP is applicable for procedure for opening of lyophilized vials of microorganisms.
Microbiologist - Quality Control
Manager –Quality Control
5.1 Prepare the required and specified media as per SOP (5 ml in 20 ml tube), for the growth of microorganisms.
5.2 Prepare the SCDA media plates and slopes as per SOP.
5.3 Wipe the Bio-safety cabinet work bench with disinfectant. Sterilize utensils like forceps, aluminium foil for culturing.
5.4 Collect all the sterile wares through pass box, remove all wrapping in Bio-safety cabinet.
5.5 Wipe the lyophile thoroughly with 70 % IPA v/v.
5.6 Scratch the surface of ampoule near the top with sterile cutter.
5.7 Snap off the top.
5.8 Remove cotton with pair of sterile forceps.
5.9 Discharge the cotton in empty sterile tube.
5.10 Transfer 1 ml sterile medium in the lyophilized powder. Suspend thoroughly on cyclo-mixture.
5.11 Streak a loop-full on agar plate as well as 0.5 ml in 5 ml medium tube. Retain remaining suspension in refrigerator.
5.12 After specified incubation, incubate pure typical colony on slant as per SOP.
5.13 Check colony morphology, as well as microscopic morphology after incubation of slant.
5.14 After confluent growth, preserve slant in refrigerator (2 to 8°C).
6.1 SOP - Standard Operating Procedure
6.2 SCDA - Soybean Casein Digest Agar
6.3 v/v - Volume/Volume
6.4 IPA - Isopropyl Alcohol
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