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SOP for Disposal of Media


Standard operating procedure of disposal of used microbiological media and culture tubes.

1.0  OBJECTIVE

       To lay down a procedure for proper disposal of used media plates and culture tubes

2.0  SCOPE

       This SOP is applicable for Media disposal

3.0  RESPONSIBILITY

       Officer/ Executive  - Quality Control

4.0  ACCOUNTABILITY

       Manager    - Quality Control

5.0  PROCEDURE

5.1  Microbial Media Plates

5.1.1  Wear rubber hand gloves and nose mask
5.1.2  Remove media content of Petri dish with the help of spatula and collect in a SS container.
5.1.3  Autoclave the SS container containing media discard at 15 psi, 121°C for 30 minutes/ validated cycle.
5.1.4  After autoclaving add 5% dettol solution to the media and dilute the autoclaved contents with tap water and drain into the sink under running tap water/ ETPplant.
5.1.5  Keep the empty glass Petri dishes for 1 hr in 5% dettol solution or savlon solution and then wash the Petri dish with water and dry them for reuse.  Disposable plates should be discarded after keeping them for 1 hr in 5% dettol or savlon solution.

5.2  CULTURE TUBES

5.2.1  Autoclave the microbial media tubes at 15 psi, 121 0C for 30 minutes/ validated cycle.
5.2.2  Drain the contents of tubes into the sink under running tap water/ETP plant.
5.2.3  Wash the empty tubes thoroughly and dry them for reuse.

5.3  ALTERNATE METHOD FOR DESTRUCTION OF DISPOSABLE PETRI  DISHES

5.3.1  Place the entire Petri dishes along with the media contents in a metal container.
5.3.2  Autoclave the container at 15 psi, 121 °C for 30 minutes/ validated cycle
5.3.3  Empty the plates into a container .
5.3.3  Dilute the contents in the container with tap water and drain into the sink under running tap water/ETP plant.
5.3.4  Empty plates should be washed.
5.3.5  After completion of Autoclave cycle for discarded media/cultured tube remove the culture tube/media from the autoclave and start a blank autoclave cycle without material to eliminate the possibility of any contamination.
5.3.6  Media preparation and media decontamination (used media discard) activities shall not be done simultaneously. 

6.0  ABBREVIATIONS:

6.1  SOP - Standard Operating Procedure
6.2  No. - Number
6.3  QA - Quality Assurance
6.4  CCF - Change control format
6.5  QC - Quality Control
6.6  NA - Not Applicable



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