Place the test solution in a separating funnel and shake with 2 quantities, each of 20 ml, and then with one 10 ml quantity of a 0.5 per w/v solution of hydroxyquinoline in chloroform.
Dilute the combined chloroform solutions to 50.0 ml with chloroform. Prepare a standard solution, in the same manner, using the prescribed reference solution. Prepare a blank in the same manner using the prescribed blank solution.
The fluorescence of the test solution (fl- f3) of the test solution is not greater than that of the standard solution (f2- f3).
Dilute the combined chloroform solutions to 50.0 ml with chloroform. Prepare a standard solution, in the same manner, using the prescribed reference solution. Prepare a blank in the same manner using the prescribed blank solution.
The fluorescence of the test solution (fl- f3) of the test solution is not greater than that of the standard solution (f2- f3).
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