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Limit Test for Aluminium

Learn how to determine the Aluminium in Pharmaceutical preparations.
Place the test solution in a separating funnel and shake with 2 quantities, each of 20 ml, and then with one 10 ml quantity of a 0.5 per w/v solution of hydroxyquinoline in chloroform.

Dilute the combined chloroform solutions to 50.0 ml with chloroform. Prepare a standard solution, in the same manner, using the prescribed reference solution. Prepare a blank in the same manner using the prescribed blank solution.

Measure the intensity of the fluorescence (204.5) of the test solution (fl), of the standard solution (f2) and of the blank (f3), using an excitant beam at 392 nm and a secondary filter with a transmission band centred on 518 nm or a monochromator set to transmit at this wavelength.

The fluorescence of the test solution (fl- f3) of the test solution is not greater than that of the standard solution (f2- f3).

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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