SOP for Measurement of Particle Size of Raw Materials Using Microscopic Method : Pharmaceutical Guidelines

SOP for Measurement of Particle Size of Raw Materials Using Microscopic Method

Standard operating procedure to determine the particle size of Raw Material microscopically.


       To lay down the procedure for measurement of particle size of raw materials using microscopic method.

2.0  SCOPE

       To measure & count the particle & particle size of raw material by visual microscopical method. 


3.1  Doing      : Technical Assistant (Q.C)/Executive
3.2  Checking : Executive / Astt. Manager  (Q.C)


       Head of the Department


5.1  Take approximately 100 mg powder in clean dry test tube and add 10 ml of paraffin oil, vortex it for 30 seconds to make uniform suspension.
5.2  Put one drop on clean, dry slide with the help of pipette. Put clean dry cover slip gently avoiding air bubbles.
5.3  Mount the slide in microscope and put micrometer scale in eye piece and focus 10X or 40X or 100X as per requirement objective.
5.4  The measurement of micrometer scale for different objective as follows.
       For  10X objective, 1 Division =10µ
       For  40X objective, 1 Division =2.5µ
       For  100X objective, 1 Division =1µ
5.5  Select 5 different microscopic field and measure total for about 200 to 300 particles.
5.6  After the completion of measurement, calculate the particle size and % particles of different particles size range as required.
5.7  The size limit and the permitted number or particles are as per individual specification.    


6.1  SOP - Standard Operating Procedure
6.2  % - percentage

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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