SOP for Cleaning of Autoclave

Standard operating procedure for cleaning of double door autoclave.

1.0 OBJECTIVE

To lay down the cleaning procedure of autoclave.

2.0 SCOPE

This procedure is applicable for cleaning of Autoclave

3.0 RESPONSIBILITY

3.1 Doing: Lab Asst./Technical Assistant
3.2 Checking: Technical Assistant/Executive

4.0 ACCOUNTABILITY

Head of the Department

5.0 PROCEDURE 

5.1 After the completion of cycle unload the autoclave.
5.2 Then shut the door II of the sterile side.
5.3 Open the non-sterile side door.
5.4 Check the chamber temperature. It should be below 50°.
5.5 Clean the chamber & trays with tap water.
5.6 Keep the chamber drain valve open
5.6 Use brush for cleaning if required.
5.7 Remove the strainer from drain hole and clean and put it back in place.
5.8 Rinse the entire chamber and trays with distilled water.
5.9 Clean the outer surface with a clean cotton cloth.
5.10 Record the cleaning in Annexure I.

6.0 ABBREVIATIONS:

SOP - Standard Operating Procedure

ANNEXURE-I
MICROBIOLOGY DEPARTMENT
CLEANING RECORDS OF AUTOCLAVE
Date
Inner side
Outer side
Cleaned By
Checked by
Remarks & Sign




























































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is a prominent Pharmaceutical Quality Assurance expert, consultant and the founder of Pharmaguideline. With over 22 years of hands-on experience in cGMP-compliant manufacturing environments, he specializes in establishing validation protocols, sterile area controls and data integrity systems. Ankur routinely interprets international regulatory frameworks (including FDA, EMA and ICH guidelines) to help global pharmaceutical professionals ensure strict regulatory compliance and operational excellence. Connect with Ankur on LinkedIn. Need Help: Ask Question

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