Preparation and Standardization of 0.001 M Sodium Dodecyl Sulphate : Pharmaceutical Guidelines

Preparation and Standardization of 0.001 M Sodium Dodecyl Sulphate

Preparation and standardisation of 0.001 M Sodium Dodecyl Sulphate using Benzethonium Chloride and Dimethyl Yellow-Oracet Blue solution as indicator.

Sodium Dodecyl Sulphate Solution Preparation

•  Dissolve 0.2884 g of sodium dodecyl sulphate, calculated with reference to the substance dried at 105° for 2 hours, in sufficient water to produce 1000 ml. 
•  Standardize the solution in the following manner.

Sodium Dodecyl Sulphate Solution Standardization

•  To 50.0 ml add 15 ml of chloroform, 10 ml of 1 M sulphuric acid and 1 ml of dimethyl yellow-oracet blue solution.
•  Titrate with 0.004 M benzethonium chloride, shaking vigorously and allowing the layers to separate after each addition, until the chloroform layer acquires a permanent clear green colour.
•  1 ml of 0.004 M benzethonium chloride is equivalent to 0.001154 g of C=H25NaO4S.

Also see: Determination of Shelf Life of Solutions in Laboratory

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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