Preparation and standardisation of 0.0005 M Dioctyl Sodium Sulphosuccinate using anhydrous Sodium Sulphate, Sodium Carbonate, Chloroform and Bromophenol Blue solution as indicator used in pharmaceutical analysis.
Dioctyl Sodium Sulphosuccinate Solution Preparation
- Dissolve 0.225 g of dicotyl sodium sulphosuccinate in warm water.
- Cool and dilute to 1000 ml with water.
- Standardize the solution in the following manner.
Dioctyl Sodium Sulphosuccinate Solution Standardization
- To 25.0 ml add 25.0 ml of a solution containing 20 % w/v of anhydrous sodium sulfate and 2 % w/v sodium carbonate, 50 ml of chloroform and 1.5 ml of bromophenol blue solution and mix.
- Titrate with 0.01 M tetrabutylammonium iodide until about 1 ml remains to be added for the end-point.
- Stopper the flask, shake vigorously for 2 minutes and continue the titration, in increments of 0.5 ml, shaking vigorously and allowing the flask to stand for about 10 seconds after each addition.
- Continue the titration until a blue color just appears in the chloroform layer.
- 1 ml of 0.01 M tetrabutylammonium iodide is equivalent to 0.004446 g of C20H37NaO7S.
Also see:
Determination of Shelf Life of Solutions in Laboratory
Dr. Ankur Choudhary is a prominent Pharmaceutical Quality Assurance expert, consultant and the founder of Pharmaguideline. With over 22 years of hands-on experience in cGMP-compliant manufacturing environments, he specializes in establishing validation protocols, sterile area controls and data integrity systems. Ankur routinely interprets international regulatory frameworks (including FDA, EMA and ICH guidelines) to help global pharmaceutical professionals ensure strict regulatory compliance and operational excellence. Connect with Ankur on LinkedIn. Need Help: Ask Question
Visitors are also reading:
No comments:
Post a Comment
Please don't spam. Comments having links would not be published.