Calibration of Heating Block : Pharmaguideline

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Calibration of Heating Block

Learn how to calibrate the heating block used for Endotoxin testing.
1. Calibration frequency once in six months
2. Operate the heating block as per SOP for operating instruction for the heating block.
3. Insert five BET tubes in a different location of wells and then add 200 ml SWFI (Sterile Water for Injection) with the help of micropipette.
4. Now insert a calibrated thermometer into the tubes containing 200 ml SWFI.

5. Set the heating block for five different temperature points firstly 30°C second 37°C third 50°C fourth 70°C and fifth 90°C.
6. After attaining the heating block temperature, noting down the temperature for 1 hour at the interval of 10 minutes for each set temperature points.
7. Compile the data and find out the temperature uniformity throughout the 1 hour period. It should be ± 1°C.
8. After calibration, immediately put the “CALIBRATION” tag duly filled with necessary information.

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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