Identification of Environmental Flora : Pharmaceutical Guidelines -->

Editable Pharmaceutical Documents in MS-Word Format


Identification of Environmental Flora

Learn how to identify the microorganisms present in production environment.

1.0 Equipment Required


2.0 Material Required

Immersion oil
Gram stain Kit

3.0 Utilities Required


4.0 Procedure

4.1 Cultural Characteristics

4.1.1 Select the unknown culture plate (microbial flora observed in sterile area environment).
4.1.2 Examine the plate for Colony characteristic as per Table - I and record the characteristic.
Table-I Colony Characteristics

4.2 Gram Staining

4.2.1 Prepare a thin smear of microbial culture observed in the sterile environment, dry in air and fix by gentle heat.
4.2.2 Hold the slide in slide rack near to ss tray and flood with Gram’s Crystal Violet (HiMedia S012) for 1 minute.
4.2.3 Wash with water and flood with Gram’s Iodine (HiMedia S013) for 1 minute.
4.2.4 Wash with water and decolourize with Gram’s Decolorizer (S032) until no further violet colour comes off.
4.2.5 Wash with water and counterstained with 0.5% safranin (HiMedia S027) for about 1 minute.
4.2.6 Wash with water, dry and observe under oil immersion objective.
4.2.7 Those bacteria that appear purple or violet color are referred as Gram Positive; those appearing pink color are described Gram Negative.
Related: Identification of Microorganisms to Species Level

Table-II Morphological Characteristic
4.2.8 After observing morphological characteristics record the observation.

5.0 Precaution

5.1 All preparation should be carried out under LAF of Microbiology room.
5.2 Use nose mask and hand gloves while working with microbial cultures.
5.3 After completion of work wash your hand with disinfectant solution.

6.0 Frequencies

Daily or as soon as colony observed in sterile area environment.

7.0 Abbreviation

LAF: Laminar Air Flow
LPG: Liquid Petrolium Gas

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
.moc.enilediugamrahp@ofni :liamENeed Help: Ask Question

No comments: Read Comment Policy ▼

Post a Comment

Please don't spam. Comments having links would not be published.

Popular Categories

QA SOPs QC SOPs Micro SOPs HVAC Production SOPs Stores SOPs Checklists Maintenance SOPs HPLC Sterile GLP Validation Protocols Water System GDP Regulatory Maintenance Calibration Warning Letters Education B.Pharmacy
Download COVID-19 SOP for Home

Follow Pharmaguideline



Editable Pharmaceutical Documents in MS-Word Format. Ready to use SOPs, Protocols, Master Plans, Manuals and more...




Pharmaceutical Updates

✔ Worldwide Regulatory Updates
✔ Pharmaceutical News Updates
✔ Interview Questions and Answers
✔ All Guidelines in One Place


Recent Posts