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# Calibration of Glassware (Class A and Class B)

Learn how to calibrate the Volumetric Glassware including flasks, pipettes and burettes.

Weigh a clean & dry volumetric flask. Calibrate the flask by filling it with recently boiled and cooled distilled water at room temperature up to the mark and weigh the flask. Determine the volume in ml by the formula;

Volume (in ml) = (Weight in g / 0.99602)
Where 0.99602 g is the weight of 1 ml water determined at temperature 25°C.
For the volumetric flasks having capacity more than 100 ml, dispense the water from 100 ml burette (class A) in multiple steps if required and note the final volume (i.e. total volume dispensed).

## Calibration of one mark pipette/ graduated pipette/ burette

Calibrate the one mark pipette / graduated pipette/ burette by filling it with recently boiled and cooled distilled water at room temperature up to the mark. Collect the water from the pipette into a clean, dry previously weighed beaker [For burette – drain the water up to the lower mark]. Take the weight of beaker with water. Check the weight of water by subtracting the wt. of empty beaker from the weight of beaker with water.
Determine the volume in ml by the formula;

Volume (in ml) = (Weight in g / 0.99602)
Where 0.99602 g is the weight of 1 ml water determined at temperature 25°C.

### Tolerance limit

Capacity in ml
5
10
25
50
100
200
250
500
1000
Tolerance
(±ml)
Class A
0.02
0.02
0.03
0.04
0.06
0.08
0.1
0.15
0.2
Class B
0.04
0.04
0.06
0.08
0.15
0.2
0.2
0.3
0.8
One mark pipette
Capacity in ml
1
2
5
10
15
20
25
50
100
Tolerance
(±ml)
Class A
0.006
0.006
0.01
0.02
0.02
0.03
0.03
0.04
0.06
Class B
0.02
0.02
0.03
0.04
0.04
0.05
0.06
0.08
0.12
Burettes
Capacity in ml
10
25
50
100
Subdivision in ml
0.05
0.05
0.1
0.1
Tolerance
(±ml)
Class A
0.01
0.03
0.05
0.1
Class B
0.02
0.06
0.1
0.2

Frequency : Initially when volumetric glassware is purchased.
Note: When the volumetric glassware does not comply with the requirement as specified above, the volumetric glassware should be returned back to the party.

### REFERENCE

Indian Pharmacopoeia.
United States Pharmacopeia.

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of pharmaguideline.com, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.

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