Identification Test of Barbiturates : Pharmaguideline -->

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Identification Test of Barbiturates

Learn how to identify the Barbiturates by Thin-layer Chromatography using Silica Gel GF254.
Determine by thin-layer chromatography, coating the plate with silica gel GF254.

Mobile phase

Shake a mixture of 80 volumes of chloroform, 15 volumes of ethanol (95 percent) and 5 volumes of strong ammonia solution. Use the lower layer.

Test solution

A 0.1 per cent w/v solution of the substance under examination in ethanol (95 percent).

Reference solution

A 0.1 per cent w/v solution of the corresponding Reference Substance in ethanol (95 per cent). Apply to the plate 10 µl of each solution. Allow the mobile phase to raise 18 cm. Remove the plate, allow to dry and examine in ultraviolet light at 254 nm. The principal spot in the chromatogram obtained with the test solution corresponds to that in the chromatogram obtained with the reference solution.

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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