Resolution Factor, Tailing Factor, Theoretical Plates and Capacity Factor in HPLC : Pharmaceutical Guidelines

Editable Pharmaceutical Documents in MS-Word Format

View

8

Resolution Factor, Tailing Factor, Theoretical Plates and Capacity Factor in HPLC

Formula and calculation for resolution factor, tailing factor, theoretical plates and capacity factor in HPLC analysis of pharmaceutical products as per usp chromatography.

Resolution factor:

Resolution factor shows the accuracy of the quantitative analysis and resolution factor should be greater than 1.5 or specified in the individual monograph. Resolution factor can be calculated by following formula:

R =               2(t2-t1)               
Calculation in Chromatography          2.70 (W1h/2+W2h/2)
W = Peak width
h = Hight
t1 = Retention time of first peak
t2 = Retention time of second peak

Tailing factor:

It should meet the requirements of the individual monograph and can be calculated by following formula:
T = W 0.05
          2F
W0.05 = Peak width at 5% high
F = Leading edge of the peak

Theoretical Plates:

The number of Theoretical Plate represents the column efficiency. It should meet the value given in the monograph. It can be calculated by the following formula:

n = 16(t)2   or  5.54x (t)2
      (W)2           (W1/2)2

Capacity factor:

K= t – 1
      tα
t = Retention time of second peak (active)
tα = Retention time of first peak (impurity)






Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
.moc.enilediugamrahp@ofni :liamENeed Help: Ask Question


Click Here

6 comments: Post Yours! Read Comment Policy ▼

  1. Good information.

    ReplyDelete
  2. Sir please explain dead volume,Void Volume and delay volume

    ReplyDelete
  3. How do i calculate peak width? ,pls

    ReplyDelete
  4. Gd information thanks

    ReplyDelete
  5. from which point we will take 5% high?

    ReplyDelete


Popular Categories

QA SOPs QC SOPs Micro SOPs HVAC Production SOPs Stores SOPs Checklists Maintenance SOPs HPLC Sterile GLP Validation Protocols Water System GDP Regulatory Maintenance Calibration Warning Letters

Follow Pharmaguideline


DOCUMENTS

PHARMACEUTICAL DOCUMENTS




Editable Pharmaceutical Documents in MS-Word Format. Ready to use SOPs, Protocols, Master Plans, Manuals and more...

View


adsbypg

Discussion - Latest Topics

Show All ❭❭Pharmaguideline Forum



GET APP FOR NEWS UPDATES

Scan to Download

Android App
Android App

Recent Posts