.
1.0 OBJECTIVE
To check the
performance of equipment for reliable and accurate results.
2.0 SCOPE
This procedure is applicable to calibrate
U.V./Visible spectrophotometer, installed in quality control department.
3.0 RESPONSIBILITY
3.1 Doing : Technical
Assistant/Executive
3.2 Checking : Executive /Manager
4.0 ACCOUNTABILITY
Head of the Department
5.0 PROCEDURE
Frequency :
Once in a three month
5.1 Operate the instrument as per respective S.O.P.
5.2 CONTROL OF ABSORBANCE
5.2.1 Weigh accurately about 60mg of potassium
dichromate previously dried at 130°C for 3 hrs. & dissolve in 0.01N
sulphuric acid to produce 1000ml.
5.2.2 Take the absorbance of the above solution at
the wavelength mentioned below and calculate A(1%; 1cm) and record the results as per the Annexure-I.
Sr.
No.
Wavelength
Limit
1
235nm
122.9-126.2
2
257nm
142.4-145.7
3
313nm
47.0-50.3
4
350nm
104.9-108.2
5.2 WAVELENGTH ACCURACY (HOLMIUM FILTER SPECTRUM)
5.3.1 Perform
baseline correction with reference to air.
5.3.2 Put the
holmium oxide filter into the cell compartment and record the absorbance
spectrum between 200nm and 600nm.
5.3.3 Check and record the maxima at the wavelengths given below. And record as per the Annexure-I.
Sr. No.
Wavelength
Limit
1
241.15nm
+ 1
nm
2
287.15nm
+ 1
nm
3
361.50nm
+ 3 nm
4
536.30nm
+ 3 nm
5.4 RESOLUTION POWER
5.4.1 Prepare
0.02% v/v solution of toluene in hexane.
5.4.2 Take spectrum and calculate the ratio of absorption at maximum at 269nm to maximum 266nm.
5.4.3 Record the
result in Annexure-I. It should not less than 1.5
5.5 LIMIT OF STRAYLIGHT
5.5.1 Prepare 1.2%
w/v solution of KCl in distilled water.
5.5.2 Take
spectrum between 190nm to
210nm and take the absorption at 200nm.
5.5.3 Record the
results in Annexure-I. It should more than 2.0.
5.5.4 The
performance of the instrument is satisfactory, if the observed wavelengths are
within the above tolerance limit.
6.0 ABBREVIATIONS
nm= Nanometer
U.V= Ultraviolet
ml=Millilitre
KCl
=Potassium chloride
SOP =
Standard operating procedure
w/v =
Weight per volume
% =
Percentage
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