SOP for Cleaning of Microbiological Laboratory Other than Clean Area

1.0 Objective

To lay down the procedure for systematic cleaning of entire Microbiology Laboratory.

2.0 Scope

This procedure is applicable to carry out daily cleaning in the Microbiology testing laboratory area other than clean area.

3.0 Responsibility

3.1 Doing: Housekeeping personnel
3.2 Checking: Executive/Manager

4.0 Accountability

Head of the Department

5.0 Procedure

5.1 SCHEDULE
A. Floor, Wall & Platforms Area Morning: Use 0.1% solution of teepol or Mocidet M300 Afternoon : as below
Sr. No.  Day             Disinfectant is used
01         Monday       2.5% Dettol
02         Tuesday      10% Solution of Aldekol-H
03         Wednesday  1% Solution of (Chlorehexidine, Gluconate 7.5%v/v+Cetrimide 15.0%w/v)
04         Thursday     2.5% Dettol
05         Friday         10% Solution of Aldekol-H
06         Saturday      1% Solution of (chlorehexidine, Gluconate 7.5%v/v+Cetrimide 15.0%w/v) (Use Hexcita brand)
For Sunday working use 0.1% solution of cleaning agent (Teepol or Macidet M300 brand)
B. Racks of Incubators & other areas
Weekly: with 70% Isopropyl alcohol
C. Glass, Doors & Window Frames
Weekly: 1. With colin solution 2. With 70% isopropyl alcohol
D. Ceiling
Weekly: with 70% Isopropyl alcohol
E. Drawers under working tables
Once in a month with dry cotton cloth
F. Switches, furniture :
Once in a month with a dry cotton cloth.
5.1 Prepare required a quantity of cleaning solution using distilled water as per schedule mentioned in as above.
5.2 Clean the entire microbiology department using following equipment
1.Floor mops 2. Cloths & Sponge (non fibre shedding) 3. Ceiling mop (non particles shedding)
5.3 Following areas are to be cleaned.
1. Chemical testing room
2. Washing room
3. Media destruction room
4. Walk in incubators: 30°-35°C and 20°-25°C
5. Media preparation room
6. Office room
7. Lobby
8. Hot area
9. Store

5.4 Follow the frequency of cleaning as per annexure.
5.5 At the end of shift drain the excess solution of cleaning agent in the drain line wash and dry the empty containers.
5.6 Mop the glasses with the help of glass wipers.
5.7 Record of cleaning record of the microbiology laboratory cleaning as per annexure.

Related: Microbial Resistance against Disinfectants

6.0 ABBREVIATIONS

LAF = Laminar Air Flow
°C = Degree centigrade
% = Percentage

ANNEXURE

CLEANING RECORD OF MICROBIOLOGICAL LABORATORY

MICROBIOLOGICAL ANALYSIS ROOM	SUNDAY DATE:	MONDAY  DATE:	TUESDAY  DATE:	WEDNESDAY DATE:	THURSDAY DATE:	FRIDAY DATE:	SATURDAY DATE:
1. Two times in a day (Floor/Wall/Platform)
a. Chemical testing room
Morning :
Afternoon :
b. Washing room
Morning :
Afternoon :
c. Media destruction room
Morning :
Afternoon :
d. Incubator (20°C-25°C) & (30°C-35°C)
Morning :
Afternoon :
e. Media preparation room
Morning :
Afternoon:
f. Office room
Morning :
Afternoon :
g. Lobby
Morning :
Afternoon :
h. Store room
Morning :
Afternoon :
i. Hot area
Morning :
Afternoon :

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