SOP for Operating Procedure of Laminar Air Flow

1.0 OBJECTIVE

To clearly define the operation of the laminar flow.

2.0 SCOPE

This procedure is applicable for laminar air flow installed in sterility area of microbiology section.

3.0 RESPONSIBILITY

3.1 Doing: Tech. Assistant (Microbiologist)
3.2 Checking: Executive/Manager

4.0 ACCOUNTABILITY

Head of the Department

5.0 PROCEDURE

5.1 Switch 'ON' the mains.
5.2 Switch ‘OFF’ U.V. light.
5.3 Switch ‘ON’ laminar air flow and light.
5.4 Check and ensure manometer reading “0” mm of water gauge before switching “ON”. Check and ensure the manometer reading between 10 to 15 mm water gauge after switching ‘ON’ the LAF and keep the record of reading.
5.5 In case the manometer reading is found out of limit, inform maintenance department for corrective action.
5.6 Clean the LAF bench with 70% IPA before use and after completion of work.
5.7 Switch ‘ON’ U.V. light after completion of work.
5.8 Switch ‘OFF’ the LAF and light after the completion of work.
5.9 Validate the LAF twice a year by the third party for DOP test/smoke Test for air velocity and for nonviable particle count.
5.10 Maintain U.V. light burning record.

6.0 ABBREVIATIONS

6.1 LAF= Laminar Air Flow
6.2 No. = Number
6.3 U.V= Ultraviolet
6.4 DOP = Dioctyl Phthalate
6.5 SOP No.= Standard operating procedure no.
6.6 % = Percentage

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