Optical Activity in Pharmaceutical Analysis : Pharmaguideline

Online GMP Courses with Certificate


Optical Activity in Pharmaceutical Analysis

Know about the optical activity, dexorotary and levorotary molecules, recemic mixtures and determination of specific optical rotation in pharmaceutical analysis.
Chiral molecules are able to rotate the plane polarized light and this quality of these molecules is known as optical activity. Optical activity is measured by polarimeter. Polarimeter has a light source, polarizing filter, sampling tube and analyzing lens. Molecules having optical activity are known as optically active molecules.

Molecules those rotate the plane polarized light to the clockwise are called dextrorotary (d) or (+) but when the molecules rotate the plane polarized light to anticlockwise are called levorotary (l) or (-). 

PolarimeterWhen both dextrorotary and levorotary isomers are found in the same amount in the mixture, it is called racemic mixture. Racemic mixtures are optically inactive.

The angle of rotation of plane-polarized light from its plane is known as the angle of rotation. All optically active molecules have unique ability to rotate the plane polarized light. Therefore they have an identical angle of rotation.

This feature of the molecule is used to identify the substances in pharmaceuticals. Sometimes substances are analyzed for assay using the optical rotation. Optical rotation depends upon the concentration of sample solution and path length through which light passes.

The polarimeter is used to determine the optical rotation. Polarimeter has a sodium light source with the wavelength of 589.3 nm and a sample tube of 1 decimeter (1d=10cm). There are many raw materials those are identified using polarimeter. The angle of rotation is recorded at 25°C and specific optical rotation of sample is calculated using following formula.

                                  100 x  α  x  100
             [α]25°D  =  -----------------------------                                   
                                 LC  x  (100 - LOD)
α = Corrected observed rotation
D = D-line of sodium light
L = Length of Polarimeter tube in dm
C = Concentration of the substance (in %)
Correction of rotation = Av. Observed reading - Av. Blank reading

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of pharmaguideline.com, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
.moc.enilediugamrahp@ofni :liamENeed Help: Ask Question

No comments:

Post a Comment

Please don't spam. Comments having links would not be published.

Popular Categories

QA SOPs QC SOPs Micro SOPs HVAC Production SOPs Stores SOPs Checklists Maintenance SOPs HPLC Sterile GLP Validation Protocols Water System GDP Regulatory Maintenance Calibration Warning Letters Education B.Pharmacy
Online Courses

Follow Pharmaguideline



Editable Pharmaceutical Documents in MS-Word Format. Ready to use SOPs, Protocols, Master Plans, Manuals and more...



Recent Posts