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HPLC Chromatography Calibration Procedure


Learn how to calibrate the High Performance Liquid Chromatography ( HPLC Chromatography ) in Pharmaceutical Quality Control.

Check HPLC chromatography (Pump) for the following :
I.  Check point  : Leakage test  (By Pressure Drop)
II.  Flow rate calibration

CHECK POINT : LEAKAGE TEST (BY PRESSURE DROP)

1.  Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2.  Place inlet tubing of the Pump in to the Water HPLC grade through suction filter.
3.  Allow mobile phase to flow for about 5 min.
4.  Block Pump outlet with the block screw.
5.  The pressure rises and on crossing the 300 bar, “ERROR P-MAX” appears on the display window. Note the time. Press “CE” key and observe the pressure drop for 5 min.
6.  After 5 min., record the pressure in calibration Log.
7.  Make entry of the column usage in the Column Usage Log Register.
8.  Make entry of the usage in to the Instrument Usage Log Register.
9.  Compare the result for its compliance against limit given in the Calibration Log and put the remark regarding the HPLC chromatography Calibration Status.
10.  In case of non-compliance, follow the Maintenance Program.

HPLC System

FLOW RATE CALIBRATION

1.  Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2.  Ensure that, the Pump is passing the “Leakage Test (By Pressure Drop)”.
3.  Keep the Drain tube in such a way that the mobile phase (Water) drops falls in to 10 ml clean, dry volumetric flask without touching the walls of the flask and start immediately the stopwatch when first drop falls in to the flask.
4.  Wait till the collected mobile phase reaches 10 ml mark of the volumetric flask and Stop the stopwatch. .
5.  Record the time required to collect the 10 ml mobile phase in calibration Log.
6.  Repeat the procedure for 1.0 ml, 1.5 ml and 2.0 ml/ min. flow rates.
7.  Repeat the step 3 to 6 but using Methanol HPLC grade as mobile phase instead of water.
8. Compare the results for its compliance against limits given in the Calibration Log and put the remark regarding the Calibration Status.
9.  Make entry of the usage of the instrument and column in the Instrument Usage Log Register and Column Usage Log Register respectively.
10.  Prepare Calibration Status Label and display on the instrument at the designated place.
11.  In case of non-compliance, follow the Maintenance Program.

REPRODUCIBILITY AND LINERAITY OF INJECTION VOLUME


Ø  Solution Mixer Preparation :

1.  Take clean and dry 50 ml volumetric flask.
2.  Pipette out 1.0 ml of Benzene and Toluene in to the clean and dry 50 ml volumetric flask.
3.  Make up the volume to 50 ml with Methanol and mix well.

Ø  Chromatographic Condition :

Column                  :           ODS C18,  (25 cm x 4.6 mm ID, 5 µm) 
Mobile Phase         :           Methanol : Water (70 : 30)
Flow Rate              :           1.0 ml/min.
Wavelength            :           254 nm
Injection Volume    :           20 µl

Calibration  :

1.  Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2.  Ensure that, the instrument is set according to the Chromatographic conditions.
3.  Follow the Instrument Operating procedure, Inject 10 µl in triplicate and record the chromatograms.
4.  Repeat the injection of above solution by injecting 15, 20, 25, 30 µl in triplicate.
5.  Take the print out of the chromatograms and attach to the Calibration Log.
6.  Record the Area and Retention times of the of the Benzene and Toluene peaks in the Calibration Log.
7.  Make entry of the usage of the instrument and column in the Instrument Usage Log Register and Column Usage Log Register respectively.
8.  Plot  the curve for area corresponding to Benzene to Toluene peaks v/s injection volume, Find out the RSD (reproducibility) and record in the Calibration Log.
9.  Find out the Correlation coefficient “r2” for each peak  at five levels and record in the Calibration Log.
10.  Compare the result for its compliance against limit given in the Calibration Log and put the remark regarding the Calibration Status.
11.  Prepare Calibration Status Label and display on the instrument at the designated place.
12.  In case of non-compliance, follow the Maintenance Program.

CHECK POINT  : D2 LAMP ENERGY CHECK (DETECTOR)

1.  Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2.  On the Detector’s display window, some values at the functions “l(nm)”, “abs(AU)”, “range(AUFS)” and “lamp” appears.
3.  On the display, previously set value blinks at  “l(nm)” function, enter the Wave length to 254 nm by pressing numerical keys.
4.  Press “Func Back” key and select “lamp” functions, enter 1 to select D2 lamp.
5.  Further press “Func Back” key till “REF EN ”  appears.
6.  Record the Reference Energy of the D2 Lamp at 254 nm in the Calibration Log.
7  Compare the result for its compliance against limit given in the Calibration Log and put the remark regarding the Calibration Status.
8.  In case of non-compliance, follow the Maintenance Program.

III.  LINEARITY OF DETECTOR RESPONSE   :

Ø  Solution Mixer Preparation :

1.  Take three clean and dry 50 ml volumetric flasks.
2.  Pipette out ml of Benzene and Toluene as per the following table in to the clean, dry 50 ml volumetric flask separately.
3.  Make up the volume to 50 ml with Methanol and mix well.
Solution (Level)
ml of Benzene to be taken
Ml of Toluene to be taken
To be Diluted with Methanol to
1
0.5
1.0
50 ml
2
1.0
1.0
50 ml
3
1.5
1.0
50 ml

Ø  Chromatographic Condition :

1.  Set each of the equipment to the following parameters as per the Equipment’s Parameter Set Up Procedure.
Column                  :           ODS C18,  (25 cm x 4.6 mm ID, 5 m)
Mobile Phase         :           Methanol : Water (70 : 30)
Flow Rate              :           1.0 ml/min.
Wavelength            :           254 nm
Injection Volume    :           20 µl


Calibration  :


1.  Ensure that, the instrument is ready for calibration and Start-up procedure is followed.
2.  Ensure that, the instrument is set according to the Chromatographic conditions.
3.  Follow the Instrument Operating procedure, Inject each of the Solutions in triplicate and record the chromatograms.
4.  Take the print out of the chromatograms and attach to the Calibration Log.
5.  Record the Area and Retention times of the of the Benzene and Toluene peaks in the Calibration Log. 
6.  Make entry of the usage of the instrument and column in the Instrument Usage Log Register and Column Usage Log Register respectively.
7.  Calculate the Area Ratio of Benzene to Toluene, Find out the Mean value of the ratios and record in the Calibration Log.
8.  Find out the Correlation coefficient “r2” from the Mean area ratio values of the three levels.and record in the Calibration Log.
8.  Plot  the curve for area ratio corresponding to Benzene to Toluene peaks v/s concentration, Find out the RSD (reproducibility) and record in the Calibration Log.
9.  Compare the result for its compliance against limit given in the Calibration Log and put the remark regarding the Calibration Status.
10.  Prepare Calibration Status Label and display on the instrument at the designated place.
11.  In case of non-compliance, follow the Maintenance Program.


CALIBRATION OF HPLC ( Liquid Chromatography ) PUMP      

Model No. :  
Make :  
Instrument Code No. :

A.  Check point  : Leakage Check Test (Pressure Drop)
P.Max
to be set
P.Max Set
Start Time
Stop Time
Total Time
Pressure Observed
Remark
300 bar






B.  Flow Rate calibration :
Flow Rate

(ml / min.)

Time required to collect 10 ml mobile phase

Limit

                         Remarks

Theoretical

(in sec.)

Actual with water

Actual with Methanol

0.5 ml

1200

 

 

1194 - 1206

 

1.0 ml

600

 

 

594 – 606

 

1.5 ml

450

 

 

443 – 457

 

2.0 ml

300

 

 

294 - 306

 













Calibration Status :  Satisfactory / Not Satisfactory
Next Calibration Due :
Calibrated By :                                                                                   Checked By :
Date :                                                                                                 Date :  

CALIBRATION OF INJECTOR

Model :                                            Make :
Instrument Code No. :

a. Preparation of solvent mixture :

Taken _____ ml (1.0 ml) of the ________ (Benzene) and ________ (Toluene) in to a _____ ml (50 ml) clean, dry volumetric flask, make up the volume with methanol, mixed well.

b. Chromatographic Condition :

Parameters

Test Condition

Applied Condition

Mobile Phase

Methanol : Water (70 : 30)

 

Column

ODS C18, (25 cm x 4.6 mm ID, 5 µm)

 

Flow Rate

1.0 ml / min.

 

Wavelength

254 nm

 


Injection volume

Injection -1

Injection -2

Injection -3

Mean

% RSD :

(Not more than 2.0%)

Benzene

Toluene

Benzene

Toluene

Benzene

Toluene

 

 

10 ml

RT

 

 

 

 

 

 

 

 

Area

 

 

 

 

 

 

 

 

15 ml

RT

 

 

 

 

 

 

 

 

Area

 

 

 

 

 

 

 

 

20 ml

RT

 

 

 

 

 

 

 

 

Area

 

 

 

 

 

 

 

 

25 ml

RT

 

 

 

 

 

 

 

 

Area

 

 

 

 

 

 

 

 

30 ml

RT

 

 

 

 

 

 

 

 

Area

 

 

 

 

 

 

 

 

Coefficient of co relations : r2 : _____________ (Limit : NLT : 0.999)
Calibration Status :  Satisfactory / Not Satisfactory
Next Calibration Due :
Calibrated By :                                                                                   Checked By :
Date :                                                                                                 Date :            

CALIBRATION OF DETECTOR

Model :  
Make :  
Instrument Code No. :

D2 LAMP ENERGY CHECK :
Wavelength to be set
Wavelength Set
Reference Energy Observed
Limit
254 nm


Not less than 200

LINEARITY OF DETECTOR RESPONSE :

a. Solution Preparation  :

Solution
ml of Benzene
ml of Toluene
in Methanol
(Level)
To be taken
Taken
To be taken
Taken
To be diluted
Diluted to
1
0.5 ml

1.0 ml

50 ml

2
1.0 ml

1.0 ml

50 ml

3
1.5 ml

1.0 ml

50 ml


Mobile Phase Preparation  : Taken ______ ml of ___________ (Methanol HPLC grade) in to a _______ ml clean, dry volumetric flask, added ____________ ml of ___________(Water HPLC grade), mixed well, allowed to cool to room temperature. Filtered through 0.45/0.22 m membrane filter, degassed for _____ min. by vacuum / sonication.



Parameters

Test Condition

Applied Condition

Mobile Phase

Methanol : Water ( 70 : 30)

 

Column

ODS C18, (25 cm x 4.6 mm ID, 5 µm)

 

Flow Rate

1.0 ml / min.

 

Wavelength

254 nm

 

Range

1.0 AUFS

 

Injection Volume

20 µl

 


Solution

(Level)

Benzene Area

Toluene Area

Area Ratio

(Benzene/Toluene)

RT

Area

RT

Area

1

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Mean :

 

 

 

 

 

% RSD  :

 

---

 

---

 

2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Mean :

 

 

 

 

 

% RSD  :

 

 

 

 

 

3

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Mean :

 

 

 

 

 

% RSD  :

 

 

 

 

 


  Linearity of Ratio : Correlation Co-efficient  r2  :

Limit :  % RSD of the Retention Time  : Not more than 2.0%

Linearity of Ratio : Correlation Co-efficient  r2  : Not less than 0.999
Calibration Status :  Satisfactory / Not Satisfactory
Next Calibration Due :
Calibrated By :                                                                                   Checked By :
Date :                                                                                                 Date : 

Related: SOP for Calibration of HPLC



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Anonymous said...

many many thanks

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