SOP for Cleaning and Operation of Bio-safety Cabinet

1.0 OBJECTIVE

To describe a procedure for cleaning and operation of the biosafety cabinet

2.0 SCOPE

2.1 This SOP is applicable for cleaning and operation of the biosafety cabinet

3.0 RESPONSIBILITY

3.1 Microbiologist - Quality Control

4.0 ACCOUNTABILITY

4.1 Manager- Quality Control

5.0 PROCEDURE

5.1 General cleaning procedure

5.1.1 Ensure that the power supply of the biosafety cabinet is switched OFF.
5.1.2 Dedust the biosafety cabinet daily externally with a clean dry cloth.
5.1.3 Once in a week remove adhered dust by wet mopping using soap solution. Afterward, wipe the surface with a clean dry cloth to remove the moisture.
5.1.4 Once in a month mop the interior surfaces with a clean dry cloth.
5.1.5 Clean the inner surface of the biosafety cabinet with 70 % IPA before and after use

5.2 Operating procedure

5.2.1 Ensure that the bio safety cabinet is properly connected to the power supply.
5.2.2 Switch ‘ON’ the main switch and then the cabinet switch.
5.2.3 Work in the bio safety cabinet for the required time and clean the equipment after work.
5.2.4 Clean the equipment with the IPA 70 %.
5.2.5 Switch ‘ON’ the UV light for thirty minutes.
5.2.6 After exposure of UV light switch ‘Off’ the main switch and then the cabinet switch.
5.2.7 Calibrate the instrument same as LAF.

6.0 ABBREVIATIONS

6.1 SOP - Standard Operating Procedure
6.2 No. - Number
6.3 QA - Quality Assurance
6.4 No. - Number
6.5 CCF - Change control format
6.6 QC - Quality Control
6.7 IPA - Isopropyl Alcohol
6.8 NA - Not Applicable

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