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SOP for Cleaning and Operation of Bio-safety Cabinet


Standard operating procedure to clean the bio safety cabinet.

1.0 OBJECTIVE

To describe a procedure for cleaning and operation of bio- safety cabinet

2.0 SCOPE

2.1 This SOP is applicable for cleaning and operation of bio- safety cabinet

3.0 RESPONSIBILITY

3.1 Microbiologist - Quality Control

4.0 ACCOUNTABILITY

4.1 Manager- Quality Control

5.0 PROCEDURE

5.1 General cleaning procedure

5.1.1 Ensure that the power supply of the bio- safety cabinet is switched OFF.
5.1.2 Dedust the bio- safety cabinet daily externally with a clean dry cloth.
5.1.3 Once in a week remove adhered dust by wet mopping using soap solution. Afterwards wipe the surface with a clean dry cloth to remove the moisture.
5.1.4 Once in a month mop the interior surfaces with a clean dry cloth.
5.1.5 Clean the inner surface of the bio- safety cabinet with 70 % IPA before and after use

5.2 Operating procedure

5.2.1 Ensure that the bio safety cabinet is properly connected to the power supply.
5.2.2 Switch ‘ON’ the main switch and then the cabinet switch.
5.2.3 Work in the bio safety cabinet for required time and clean the equipment after work.
5.2.4 Clean the equipment with the IPA 70 %.
5.2.5 Switch ‘ON’ the UV light for thirty minutes.
5.2.6 After exposure of UV light switch ‘Off’ the main switch and then the cabinet switch.
5.2.7 Calibrate the instrument same as LAF.

6.0 ABBREVIATIONS

6.1 SOP - Standard Operating Procedure
6.2 No. - Number
6.3 QA - Quality Assurance
6.4 No. - Number
6.5 CCF - Change control format
6.6 QC - Quality Control
6.7 IPA - Isopropyl Alcohol
6.8 NA - Not Applicable
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
Email: .moc.enilediugamrahp@ofni Need Help: Ask Question


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