# Importance and Determination of F0 Value in Sterilization

Learn how F0 value is determined for sterilization using formula including Z value.
Generally sterilization is done at 121 °C and it is a standard temperature for sterilization. It we want to sterilize the material at the temperature other than 121 °C then we must change the time of sterilization.

Some materials are heat sensitive and cannot be sterilized at 121 °C. F0 value is used to determine the exposure time of material for sterilization at particular temperature. F0 value is the time in minute for the specified temperature that gives the same thermal lethality as at 121 °C in one minute.

Related: Determination of Z-Value

It explains that temperature is not an important factor in thermal sterilization but lethality is important. Time of sterilization can be changed (decrease or increase) according to the lethality of the temperature.

F0 = Δt x 10 (T –121/ Z)

Where,
T= Temperature for Sterilization
Δt = 60 Sec. = 1min.
Z = 10°C

For Example:
If we want to sterilize any material at 115 °C then we have to calculate F0 Value –
F0 = 1 x 10 (115–121/ 10)
F0 = 1 x 10 (–6/ 10)
F0 = 1 x 10 (–0.6)
F0 = 3.98 minutes

It shows that that the thermal lethality of 1 minute at 121 °C is equal to the lethality of 3.98 minutes at 115 °C.

Also see: Log Reduction (D Value) and Sterility Assurance Level (SAL)

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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