SOP for Cleaning and Sanitization of Microbiology Section with Disinfectants

1.0 OBJECTIVE

To lay down the procedure for Cleaning and Sanitation of Microbiology Laboratory.

2.0 SCOPE

This SOP shall be applicable to Quality Control Dept.

3.0 RESPONSIBILITY

Microbiologist

4.0 ACCOUNTABILITY

Head Quality Control Department

5.0 PROCEDURE

5.1 Safety Precautions

5.1.1 Use proper safety apparels such as gowns, rubber hand gloves and safety goggles during preparation and use of Disinfectants.
5.1.2 Always add disinfectant to water while diluting.

5.2 Disinfectant Solutions

2.5 % Dettol
5.0 % Lysol
7.0 % Savlon
70% Isopropyl Alcohol (For disinfecting hands and cleaning of LAF)
Prepare quantities of disinfectant solutions sufficient for one-day cleaning.

5.3 Procedure For Preparation of Disinfectant Solution

5.3.1 2.5% Dettol (For 1 Liter)
Take 500 ml of Purified water add 25 ml of Dettol and make-up volume to 1000 ml with Purified water. Mix well and use within a day.
5.3.2 5.0 % Lysol (For 1 Liter)
Take 500 ml of Purified water add 50 ml of Savlon and make-up the volume to 1000 ml with Purified water. Mix well & use within a day.
5.3.3 7 % Savlon (For 1 Liter)
Take 500 ml of Purified water add 70 ml of Lysol & make up the volume to 1000 ml with Purified water. Mix well & use within a day.
5.3.4 70% IPA (For 1 Liter)
Take 100 ml of Purified water and add 700 ml of IPA & make-up to 1000 ml. Mix well.

5.4 General Instructions

5.4.1 Perform the cleaning operation in the following order.5.4.1.1 Microbiology Testing Area and Change Rooms5.4.1.2 Autoclave room5.4.1.3 Media Preparation room
5.4.2 All disinfectant solutions shall be prepared freshly.
5.4.3 Use Purified Water for preparing disinfectant solutions.
5.4.4 Use Clean dusters for cleaning of Microbiology Testing area and its change rooms , Autoclave room and Media Preparation room.
5.4.5 Use the disinfectant solutions alternatively on weekly basis.
5.4.6 Record the preparation of disinfectant.
Related: SOP for Guidelines for Working in Microbiology Laboratory

5.5 Daily Cleaning Procedure

Frequency: Once in a day.
5.5.1 After completion of day’s work clean the floor using a nylon brush / non-shedding duster.
5.5.2 Mop the area in uni-direction.
5.5.3 Subsequently mop the floor using non-shedding wiping mops dipped in the disinfectant.
5.5.4 After every moping clean the non-shedding wiping mops in purified water. Then again dip in the scheduled disinfectant and use.
5.5.5 Maintain records of daily cleaning in cleaning record of Microbiology department.

5.6 Weekly Cleaning Procedure

Frequency: Once inWeek.
5.6.1 Clean the areas in the following order with non-shedding duster.5.6.1.1 Ceiling and fixtures5.6.1.2 Walls
5.6.2 Subsequently, mop the area as in the order given using a non-shedding mop dipped in any one of the disinfectant solutions.
5.6.3 Maintain records of weekly cleaning in Cleaning record of Microbiology department.
Daily sanitation schedule of microbiological testing area.

Week day	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
Mop	Dettol 2.5%	Lysol 5.0%	Savlon 7.5%	Lysol 5.0%	Dettol 2.5%	Lysol 5.0%	Savlon 7.5%

6.0 ABBREVIATIONS

6.1 SOP: Standard Operating Procedure
6.2 QA: Quality Assurance
6.3 QC: Quality Control
6.4 Dept.: Department

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