SOP for Operation of Karl Fischer Apparatus : Pharmaguideline -->

Editable Pharmaceutical Documents in MS-Word Format


SOP for Operation of Karl Fischer Apparatus

Standard operating procedure to operate the Karl Fischer apparatus used to determine the moisture content.


To lay down the procedure for operation of Karl Fischer apparatus.


This SOP shall be applicable to Quality Control Dept.


Quality Control Supervisor


Sr. Manager Quality Assurance


5.1 Take about 150 ml of Karl Fischer reagent. The glassware used should be well dried as otherwise, the reagent will become useless due to moisture.
5.2 Put the Karl Fischer reagent in the reservoir bottle provided at the back and place the stopper immediately after addition of Reagent.
5.3 Remove large rubber cork and add some methanol to the moisture beaker to such an extent that the metal wires of the sensors in the beaker are fully immersed. Replace the cork immediately.
5.4 Connect leads of the relay to the small three-pin socket on the titrator. Then connect the sensor wires to a socket provided on the instruments. Adjust the speed of the magnetic stirrers such that the wire ends of the sensor do not touch the stirred and at the same time sensors should not be exposed to air.
5.5 Put the dispensing burette on top of Karl Fischer Reagent bottle and close it properly.
5.6 Connect the titrator to the main supply. Veego Home screen will be displayed.
5.7 Rotate Stirrer knob provided on the right side of the instrument. Stirrer will be on.
Adjust the speed of the magnetic stirrer in such a way that the wired ends of the sensors do not touch the stirrer and at the same time sensors should not be exposed to air.
5.8 Press Start key. Instrument Starts calculating and neutralizing the water previously present in the methanol by adding Karl Fischer reagent. Once methanol is neutralized repeat the same procedure till KF reading for methanol comes to zero.
5.9 Weigh the sample for water content is to be determined. Press start key and add sample to methanol for water determination. KF reading will be displayed.
5.10 Press “Smpl” key and add the weight of sample taken for analysis. Press enter key and then “%age, PPM, mgH2O” key to display water content present in the sample.


6.1 SOP: Standard Operating Procedure
6.2 QA: Quality Assurance
6.3 QC: Quality Control
6.4 Dept.: Department
6.5 KF: Karl Fischer
Get ready to use editable documents in MS-Word FormatView List

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
.moc.enilediugamrahp@ofni :liamENeed Help: Ask Question

3 comments: Post Yours! Read Comment Policy ▼

Please don't spam. Comments having links would not be published.

Popular Categories

QA SOPs QC SOPs Micro SOPs HVAC Production SOPs Stores SOPs Checklists Maintenance SOPs HPLC Sterile GLP Validation Protocols Water System GDP Regulatory Maintenance Calibration Warning Letters Education B.Pharmacy
Resume Pro Video Tutorials
Subscribe on Youtube


Show All ❭❭Jobs by PharmaJobs

Follow Pharmaguideline



Editable Pharmaceutical Documents in MS-Word Format. Ready to use SOPs, Protocols, Master Plans, Manuals and more...




Pharmaceutical Updates

✔ Worldwide Regulatory Updates
✔ Pharmaceutical News Updates
✔ Interview Questions and Answers
✔ All Guidelines in One Place


Recent Posts