Preparation and standardisation of 0.05 M Bromine solution using Sodium Thiosulphate and 3 ml of Starch solution as indicator that is used in chemical analysis of pharmaceutical substances.
Bromine Solution Preparation
- Dissolve 3 g of potassium bromate and 15 g of potassium bromide in sufficient water to produce 1000 ml.
- Standardize the solution in the following manner.
Bromine Solution Standardization
- Pipette 25.0 ml of the solution into a 500 ml iodine flask and dilute with 120 ml of water.
- Add 5 ml of hydrochloric acid, insert the stopper in the flask and shake it gently.
- Add 5 ml of potassium iodide solution, again insert the stopper and allow it to stand for 5 minutes in the dark.
- Titrate the liberated iodine with 0.1 M sodium thiosulphate using 3 ml of starch solution, added towards the end of the titration, as an indicator.
- 1 ml of 0.1 M sodium thiosulphate is equivalent to 0.01598 g of Br2.
- Store in dark amber-colored, glass-stoppered bottles.
Also see:
Determination of Shelf Life of Solutions in Laboratory
Dr. Ankur Choudhary is a prominent Pharmaceutical Quality Assurance expert, consultant and the founder of Pharmaguideline. With over 22 years of hands-on experience in cGMP-compliant manufacturing environments, he specializes in establishing validation protocols, sterile area controls and data integrity systems. Ankur routinely interprets international regulatory frameworks (including FDA, EMA and ICH guidelines) to help global pharmaceutical professionals ensure strict regulatory compliance and operational excellence. Connect with Ankur on LinkedIn. Need Help: Ask Question
Visitors are also reading:
No comments:
Post a Comment
Please don't spam. Comments having links would not be published.