Preparation and Standardization of 0.05 M Bromine : Pharmaguideline

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Preparation and Standardization of 0.05 M Bromine

Preparation and standardisation of 0.05 M Bromine solution using Sodium Thiosulphate and 3 ml of Starch solution as indicator that is used in chemical analysis of pharmaceutical substances.

Bromine Solution Preparation

  • Dissolve 3 g of potassium bromate and 15 g of potassium bromide in sufficient water to produce 1000 ml.
  • Standardize the solution in the following manner.

Bromine Solution Standardization

  • Pipette 25.0 ml of the solution into a 500 ml iodine flask and dilute with 120 ml of water.
  • Add 5 ml of hydrochloric acid, insert the stopper in the flask and shake it gently.
  • Add 5 ml of potassium iodide solution, again insert the stopper and allow it to stand for 5 minutes in the dark. 
  • Titrate the liberated iodine with 0.1 M sodium thiosulphate using 3 ml of starch solution, added towards the end of the titration, as an indicator.
  • 1 ml of 0.1 M sodium thiosulphate is equivalent to 0.01598 g of Br2.
  • Store in dark amber-colored, glass-stoppered bottles.
Also see: Determination of Shelf Life of Solutions in Laboratory

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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