Calibration of Digital Polarimeter : Pharmaceutical Guidelines

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Calibration of Digital Polarimeter

Learn how to calibrate the Digital Polarimeter using Sucrose solution.
Ensure that all the connections of the instrument are proper.
Turn main power supply ON.
Prepare a solution of different concentrations by using sucrose A.R. grade previously dried at 105°C for 1 hour as follows:
Sr. No.
Concentration of Sucrose (in %)
Weight Taken (in g)
Dilution Volume with water (ml)
1
10
10.0
100
2
20
20.0
100
3
30
30.0
100
4
40
40.0
100
5
50
50.0
100

Enter the actual concentration with respect to the weight taken in MODE: 1. Measure the angular rotation obtained, calculate RSD% for 5 readings obtained in each concentration, calculate the correlation coefficient for different concentration [Refer Annexure 1].
Checks reading obtained with annexure for acceptance criteria.

Precautions

Check solution temperature.
While filling polarimeter tube due care should be taken to avoid air bubble in the path of the light source.
While using tubes with removable end plates fitted with gasket tighten end plate only enough to ensure a leak-proof seal between the end plate and the body of the tube.
Liquids and solutions of solid must be clear.
Frequency – Once a month
Related: Determination of Optical Rotation and Specific Optical Rotation

ANNEXURE-1
Sr. No.
Concentration of Sucrose (In %)
Optical Rotation (µ)
At 25°C
Tolerance
Minimum
Maximum
1.
10.0
13.33°
13.18°
13.40°
2.
20.0
26.61°
26.36°
26.80°
3.
30.0
39.86°
39.54°
40.20°
4.
40.0
53.06°
52.72°
53.60°
5.
50.0
66.23°
65.90°
67.00°

ACCEPTANCE CRITERIA FOR RSD %: Not more than 1.0%
ACCEPTANCE CRITERIA FOR CORRELATION COEFFICIENT: Not less than 0.995
REFERENCE
Indian Pharmacopoeia & United States Pharmacopoeia





Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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