Calibration of Melting Point Apparatus : Pharmaceutical Guidelines

Calibration of Melting Point Apparatus

Learn how to calibrate the Melting Point Apparatus using Vanillin, Acetanilide, Urea, Sulphanilamide, Caffeine and Diclofenac Sodium.


1.  Ensure that all the connections of the instrument are proper.
2.  Operate the instrument as per the operating instructions and determine the melting point of the following reference substances. 
3.  Take the reference substances from lowest range to highest range at a time for calibration.

Reference Substances
Standard Range
81.0°C – 83.0°C
114.0°C – 116.0°C
Urea (finely crushed)
132.0°C – 135.0°C
164.5°C – 166.5°C
Caffeine (dried at 100°C)
234.0°C – 237.0°C
Diclofenac Sodium
277.0°C – 283.0°C

Acceptance criteria

The melting point of the reference substances should be within the standard range.


Once in a month

Maintenance / Repairs

When the instrument does not comply with the requirement / tolerance range specified above, the instrument should be labeled “OUT OF CALIBRATION” and should be repaired / serviced.
After repair / maintenance, calibrate the instrument.

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
[email protected] :liamENeed Help: Ask Question

Click Here

No comments: Read Comment Policy ▼

Post a Comment


Show All ❭❭Jobs by PharmaJobs

Write a Powerful CV

Click Here

Advertise Here

Get App for News Updates

Scan to Download

Android App
Android App

Recent Posts